Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage

详细信息    查看全文

• 作者：A. A. Toymentseva ; I. V. Danilova…
• 关键词：Bacillus pumilus 3 ; 19 ; expression vector ; subtilisin ; like protease ; glutamylendopeptidase ; metalloendopeptidase ; affinity chromatography ; biological activity
• 刊名：Russian Journal of Bioorganic Chemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：42
• 期：1
• 页码：53-58
• 全文大小：411 KB
• 参考文献：1.Danilova, Yu.V., Cheremin, A.M., Zamaleeva, A.I., Mardanova, A.M., Zamalyutdinova, N.M., and Sharipova, M.R., Klet. Transplantol. Tkan. Inzhener., 2012, vol. 7, pp. 49–51.
  2.Hsu, R.L., Lee, K.T., Wang, J.H., Lee, L.Y., and Chen, R.P., Agric. Food Chem., 2009, vol. 28, pp. 503–508.CrossRef
  3.Shamsutdinov, T.R., Balaban, N.P., Mardanova, A.M., Danilova, Yu.V., Rudenskaya, G.N., and Sharipova, M.R., Bioorg. Khim., 2008, vol. 34, pp. 322–326.PubMed
  4.Rudakova, N.L., Balaban, N.P., and Danilova, Yu.V., Biokhimiya, 2010, vol. 75, pp. 1462–1470.
  5.Herzberg, C., Weidinger, L.A., Dorrbecker, B., Hubner, S., Stulke, J., and Commichau, F.M., Proteomics, 2007, vol. 7, pp. 4032–4035.CrossRef PubMed
  6.Msadek, T., Kunst, F., Klier, A., and Rapoport, G., Bacteriology, 1991, vol. 173, pp. 2366–2377.
  7.Vellanoweth, R.L. and Rabinowitz, J.C., Mol. Microbiol., 1992, vol. 6, pp. 1105–1114.CrossRef PubMed
  8.Ma, J., Campbell, A., and Karlin, S.J., Bacteriology, 2002, vol. 184, pp. 5733–5745.CrossRef
  9.Mikhailova, E.O., Mardanova, A.M., Balaban, N.P., Rudenskaya, G.N., Ilyinskaya, O.N., and Sharipova, M.R., Biochemistry, 2009, vol. 74, pp. 308–315.PubMed
  10.Sabirova, A.R., Rudakova, N.L., Balaban, N.P., Ilyinskaya, O.N., Demidyuk, I.V., Kostrov, S.V., Rudenskaya, G.N., and Sharipova, M.R., FEBS Lett., 2010, vol. 584, pp. 4419–4425.CrossRef PubMed
  11.Seeman, P. and Seeman, N., Synapse, 2011, vol. 65, pp. 1289–1297.CrossRef PubMed
  12.Esteras-Choro, A., Serrano, L., and Lopes de la Raz, M., Proc. Natl. Acad. Sci. U.S.A., 2005, vol. 102, pp. 16672–16677.CrossRef
  13.Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., New York, Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989.
  14.Anagnostopolous, C. and Spizizen, J., Bacteriology, 1961, vol. 81, pp. 741–746.
  15.Lyublinskaya, L.A., Khaidu, I., and Balandina, G.N., Bioorg. Khim., 1987, vol. 13, pp. 748–753.
  16.Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.CrossRef PubMed
  
• 作者单位：A. A. Toymentseva (1)
  I. V. Danilova (1)
  A. O. Tihonova (1)
  M. R. Sharipova (1)
  N. P. Balaban (1)
  
  1. Kazan Federal University, Kazan, 420008, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Organic Chemistry
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-330X

文摘

Using the expression vector pGP382 containing a constitutive promoter (P _degQ36 ) and an affinity tag (Strep-tag), we have obtained highly purified recombinant Bacillus pumilus 3-19 proteinases with different substrate specificities: glutamylendopeptidase (GseBp), subtilisin-like protease (AprBp), and metalloendopeptidase (MprBp). The products of the hydrolysis of the ß-amyloid peptide by the bacterial proteases from B. pumilus have been studied. The findings on the potential of the practical application of these bacterial enzymes as the agents preventing the development of the Alzheimer’s disease are presented. Keywords Bacillus pumilus 3-19 expression vector subtilisin-like protease glutamylendopeptidase metalloendopeptidase affinity chromatography biological activity