Thermostable Xanthine Oxidase Activity from Bacillus pumilus RL-2d Isolated from Manikaran Thermal Spring: Production and Characterization

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• 作者：Nirmal Kant Sharma ; Shikha Thakur ; Neerja Thakur ; Savitri…
• 关键词：Bacillus pumilus ; Xanthine oxidase ; Thermophilic microorganisms ; Thermostability
• 刊名：Indian Journal of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：56
• 期：1
• 页码：88-98
• 全文大小：700 KB
• 参考文献：1.Apaya KL, Chichioco-Hernandez CL (2011) Xanthine oxidase inhibition of selected Philippine medicinal plants. J Med Plants Res 5:289–292
  2.Xin Y, Yang H, Xia X, Zhang L, Zhang Y, Cheng C, Wang W (2012) Expression, purification and partial characterization of a xanthine oxidase (XOD) in Arthrobacter sp. Process Biochem 47:1539–1544. doi:10.​1016/​j.​procbio.​2012.​01.​007 CrossRef
  3.Papapostolou I, Georgiou CD (2010) Superoxide radical is involved in the sclerotial differentiation of filaments phytopathogenic fungi: identification of a fungal xanthine oxidase. Fungal Biol 114:387–395. doi:10.​1016/​j.​funbio.​2010.​01.​010 CrossRef PubMed
  4.Agarwal A, Banerjee UC (2009) Screening of xanthine oxidase producing microorganisms using nitroblue tetrazolium based colorimetric assay method. Open Biotechnol J 3:46–49. doi:10.​2174/​1874-0707/​09 CrossRef
  5.George J, Struthers AD (2008) The role of urate and xanthine oxidase inhibitors in cardiovascular disease. Cardiovasc Ther 26:59–64. doi:10.​1111/​j.​1527-3466.​2007.​00029 PubMed
  6.Tanigaki N, Furukawa K, Sogabe Y, Emi S (1993) Thermostable xanthine oxidase from Arthrobacter luteus. United States Patent 518525
  7.Pauff JM, Zhang J, Bell CE, Hille R (2008) Substrate orientation in xanthine oxidase: crystal structure of enzyme in reaction with 2-hydroxy-6-methylpurine. J Biol Chem 283:4818–4824. doi:10.​1074/​jbc.​M707918200 CrossRef PubMed
  8.Zhang Y, Xin Y, Yang H, Zhang L, Xia X, Tong Y, Chen Y, Ma L, Wang W (2012) Novel affinity purification of xanthine oxidase from Arthrobacter M3. J Chromatogr B 906:19–24. doi:10.​1016/​j.​jchromb.​2012.​08.​007 CrossRef
  9.Dolmaci N, Cete S, Arslan F, Yaşar A (2012) An amperometric biosensor for fish freshness detection from xanthine oxidase immobilized in polypyrrole polyvinylsulphonate film. Artif Cells Nanomed Biotechnol 40:275–279. doi:10.​3109/​10731199.​2011.​646410
  10.Sathisha KR, Khanum SA, Chandra JNNS, Ayisha F, Balaji S, Marathe GK, Gopal S, Rangappa KS (2011) Synthesis and xanthine oxidase inhibitory activity of 7-methyl-2-(phenoxymethyl)-5H-[1,3,4]thiadiazolo[3,2-a]pyrimidin-5-one derivatives. Bioorg Med Chem 19:211–220. doi:10.​1016/​j.​bmc.​2010.​11.​034 CrossRef PubMed
  11.Kalra S, Jena G, Tikoo K, Mukhopadhyay AK (2007) Preferential inhibition of xanthine oxidase by 2-amino-6-hydroxy-8-mercaptopurine and 2-amino-6-purine thiol. BMC Biochem 8:1–11. doi:10.​1186/​1471-2091-8-8 CrossRef
  12.Singh AK, Tripathi BM, Sahay H, Singh RN, Kaushik R, Saxena AK, Arora DK (2010) Biochemical and molecular characterization of thermo-alkali tolerant xylanase producing bacteria from thermal springs of Manikaran. Indian J Microbiol 50:S2–S9. doi:10.​1007/​s12088-010-0071-4 CrossRef
  13.Kumar D, Verma R, Sharma P, Rana A, Sharma R, Prakash C, Bhalla TC (2010) Production and partial purification of xylanase from a new thermophilic isolate. Biol Forum Int J 2:83–87
  14.Mahajan J, Kumar R, Kaur R, Arora S (2012) Exploration of xanthine oxidase producing microorganisms from hypoxanthine supplemented soil and their inhibitors from plants employing nitrobluetetrazolium based colorimetric assay method. GARJEST 1:010–013
  15.Nakanishi T, Machida Y (1982) Method and test composition for the determination of the substrate for xanthine oxidase. United States Patent 4341868A
  16.Dwivedi V, Kumari K, Gupta SK, Kumari R, Tripathi C, Lata P, Niharika N, Singh AK, Kumar R, Nigam A, Garg N, Lal R (2015) Thermus parvatiensis RLT sp. Nov, isolated from a hot water spring, located atop the Himalayan ranges at Manikaran, India. Indian J Microbiol. doi:10.​1007/​s12088-015-0538-4 PubMed
  17.Mondal MS, Sau AK, Mitra S (2000) Mechanism of the inhibition of milk xanthine oxidase activity by metal ions: a transient kinetic study. Biochim Biophys Acta 1480:302–310. doi:10.​1016/​S0167-4838(00)00084-4 CrossRef PubMed
  18.Gardlik G, Rajagopalan KV (1990) The state of reduction of molybdopterin in xanthine oxidase and sulfite oxidase. J Biol Chem 265:13047–13054PubMed
  
• 作者单位：Nirmal Kant Sharma (1)
  Shikha Thakur (1)
  Neerja Thakur (1)
  Savitri (1)
  Tek Chand Bhalla (1)
  
  1. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, Himachal Pradesh, 171005, India
  
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Springer India
• ISSN：0973-7715

文摘

Xanthine oxidase is an important enzyme of purine metabolism that catalyzes the hydroxylation of hypoxanthine to xanthine and then xanthine to uric acid. A thermostable xanthine oxidase is being reported from a thermophilic organism RL-2d isolated from the Manikaran (Kullu) hot spring of Himachal Pradesh (India). Based on the morphology, physiological tests, and 16S rDNA gene sequence, RL-2d was identified as Bacillus pumilus. Optimization of physiochemical parameters resulted into 4.1-fold increase in the xanthine oxidase activity from 0.051 U/mg dcw (dry cell weight) to 0.209 U/mg dcw. The xanthine oxidase of B. pumilus RL-2d has exhibited very good thermostability and its t_1/2 at 70 and 80 °C were 5 and 1 h, respectively. Activity of this enzyme was strongly inhibited by Hg2+, Ag+ and allopurinol. The investigation showed that B. pumilus RL-2d exhibited highest xanthine oxidase activity and remarkable thermostability among the other xanthine oxidases reported so far. Keywords Bacillus pumilus Xanthine oxidase Thermophilic microorganisms Thermostability