Piper betle leaf extract affects the quorum sensing and hence virulence of Pseudomonas aeruginosa PAO1

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• 作者：Siraj Datta ; Debanjan Jana ; Tilak Raj Maity ; Aveek Samanta ; Rajarshi Banerjee
• 关键词：Anti Quorum Sensing properties ; Piper betle leaf extract ; Pseudomonas aeruginosa ; Biofilm production ; Pyocyanin assay ; Mobility pattern
• 刊名：3 Biotech
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：1
• 全文大小：799 KB
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• 作者单位：Siraj Datta (1)
  Debanjan Jana (1)
  Tilak Raj Maity (1)
  Aveek Samanta (1)
  Rajarshi Banerjee (1)
  
  1. Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal, 721657, India
  
• 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-5738

文摘

Quorum sensing (QS) plays an important role in virulence of Pseudomonas aeruginosa, blocking of QS ability are viewed as viable antimicrobial chemotherapy and which may prove to be a safe anti-virulent drug. Bioactive components from Piper betle have been reported to possess antimicrobial ability. This study envisages on the anti-QS properties of ethanolic extract of P. betle leaf (PbLE) using P. aeruginosa PAO1 as a model organism. A marked reduction in swarming, swimming, and twitching ability of the bacteria is demonstrated in presence of PbLE. The biofilm and pyocyanin production also shows a marked reduction in presence of PbLE, though it does not affect the bacterial growth. Thus, the studies hint on the possible effect of the bioactive components of PbLE on reducing the virulent ability of the bacteria; identification of bioactive compounds should be investigated further. Keywords Anti Quorum Sensing properties Piper betle leaf extract Pseudomonas aeruginosa Biofilm production Pyocyanin assay Mobility pattern