Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves

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• 作者：A. M. Selim ; M. M. Elhaig ; I. Zakaria ; A. Ali
• 关键词：Bacteriology ; Clostridium perfringens ; Multiplex PCR ; Seasonal prevalence
• 刊名：Tropical Animal Health and Production
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：49
• 期：1
• 页码：201-205
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Zoology; Veterinary Medicine/Veterinary Science;
• 出版者：Springer Netherlands
• ISSN：1573-7438
• 卷排序：49

文摘

Clostridium perfringens is considered one of the important causes of calf diarrhea. Two hundred and twenty-seven clinical samples from newly born and dead diarrheic calves were examined bacteriologically and by PCR. Bacterial culture identified C. perfringens in 168 of 227 samples. A total of 144 of these isolates were lecithinase positive, indicating C. perfringens Type A. In addition, 154 isolates were positive by alpha toxin encoding gene-PCR assay. This study showed high agreement between the results of bacteriology and multiplex PCR. The multiplex PCR typed all isolates that were typed as C. perfringens Type A through bacteriologic methods, but ten samples that were lecithinase negative were positive in the multiplex PCR. The study showed the highest occurrence of C. perfringens Type A isolations from calves during the winter and autumn compared with other seasons.