Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans

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• 作者：Shenghao Liu (1) (2)
  Jing Wang (1)
  Bailin Cong (2)
  Xiaohang Huang (2)
  Kaoshan Chen (1)
  Pengying Zhang (1)
  
  1. National Glycoengineering Research Center ; College of Life Science ; Shandong University ; 27 Shandanan Road ; Jinan ; 250100 ; People鈥檚 Republic of China
  2. Key Laboratory of Marine Bioactive Substance ; The First Institute of Oceanography ; State Oceanic Administration ; Qingdao ; 266061 ; People鈥檚 Republic of China
  
• 关键词：Cold adaptation ; Pohlia nutans ; HSP70 ; Psychrophile ; Abiotic stress
• 刊名：Polar Biology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：37
• 期：8
• 页码：1145-1155
• 全文大小：1,914 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Ecology
  Oceanography
  Microbiology
  Plant Sciences
  Zoology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2056

文摘

Under stress conditions, the mitochondrial respiratory chain generates reactive oxygen species that cause oxidative damage to lipids, proteins and nucleic acids. Mitochondrial chaperones are essential for repairing denatured proteins in plants confronted with diverse stresses. However, their functions have not been well characterized in lower plants such as bryophytes. Here, we cloned a heat-shock 70 protein gene (designated PnHSP70) from the Antarctic moss Pohlia nutans and investigated its transcription levels under stress conditions. The PnHSP70 cDNA encoded a polypeptide of 678 amino acids with an ATPase domain, a substrate peptide-binding domain and a C-terminal domain. Sequence alignment and phylogenetic analysis showed that PnHSP70 was homologous to other known mitochondrial HSP70 proteins. In addition, subcellular localization analysis in which the transient expression of PnHSP70-green fluorescent protein was observed in Arabidopsis thaliana mesophyll protoplasts revealed that PnHSP70 was targeted to mitochondria. Real-time PCR analysis showed that low or high temperature, salinity, drought or PEG treatments as well as UV-B radiation could induce up-regulation of PnHSP70 expression levels. The plant hormones abscisic acid and jasmonic acid also up-regulated the expression levels of PnHSP70. Together, our results revealed that PnHSP70, as a mitochondrial chaperone, might play an important role in the adaptation of the Antarctic moss P. nutans to the polar environment.