文摘
Stroma, envelope and thylakoid membranes were prepared from chloroplasts isolated from leaves of Beta vulgaris. Out of total plastidic protochlorophyllide, envelope membranes contained 1.5 % , thylakoids had the maximum 98.48 % and stroma had a trace fraction of 0.02 % . Distribution of the Mg-protoporphyrin IX and its monoester was 89.0 % in thylakoids, 10.0 % in stroma and 1.0 % in envelope. A substantial fraction (33.77 % ) of plastidic protoporphyrin IX was partitioned into stroma. Envelope contained 0.66 % and thylakoids had 65.57 % of the total plastidic protoporphyrin IX pool. The proportion of monovinyl and divinyl forms of protochlorophyllide was almost similar in intact plastid, thylakoids, and outer and inner envelope membranes suggesting a tight regulation of vinyl reductase enzyme. The significance of differential distribution of chlorophyll biosynthetic intermediates among thylakoids, envelope and stroma is discussed.