OsBAK1 is involved in rice resistance to Xanthomonas oryzae pv. oryzae PXO99

详细信息    查看全文

• 作者：Hualan Liao ; Xiaorong Xiao ; Xiuqiong Li ; Yan Chen ; Xiumei Fu…
• 关键词：Rice ; OsBAK1 ; Overexpression ; RNAi ; Pathogen resistance ; PXO99
• 刊名：Plant Biotechnology Reports
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：10
• 期：2
• 页码：75-82
• 全文大小：1,242 KB
• 参考文献：Agrawal GK, Rakwal R, Jwa NS (2000) Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals. Biochem Biophys Res Commun 278(2):290–298CrossRef PubMed
  Akira S, Uematsu S, Takeuchi O (2006) Pathogen recognition and innate immunity. Cell 124(4):783–801CrossRef PubMed
  Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J (2002) MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415(6875):977–983CrossRef PubMed
  Belkhadir Y, Chory J (2006) Brassinosteroid signaling: a paradigm for steroid hormone signaling from the cell surface. Science 314(5804):1410–1411CrossRef PubMed
  Boller T, Felix G (2009) A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annu Rev Plant Biol 60:379–406CrossRef PubMed
  Chinchilla D, Zipfel C, Robatzek S, Kemmerling B, Nurnberger T, Jones JD, Felix G, Boller T (2007) A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature 448(7152):497–500CrossRef PubMed
  Chisholm ST, Coaker G, Day B, Staskawicz BJ (2006) Host-microbe interactions: shaping the evolution of the plant immune response. Cell 124(4):803–814CrossRef PubMed
  Chittoor JM, Leach JE, White FF (1997) Differential induction of a peroxidase gene family during infection of rice by Xanthomonas oryzae pv. oryzae. Mol Plant-microbe Interact MPMI 10(7):861–871CrossRef PubMed
  Felix G, Boller T (2003) Molecular sensing of bacteria in plants. The highly conserved RNA-binding motif RNP-1 of bacterial cold shock proteins is recognized as an elicitor signal in tobacco. J Biol Chem 278(8):6201–6208CrossRef PubMed
  Gomez-Gomez L, Boller T (2000) FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. Mol Cell 5(6):1003–1011CrossRef PubMed
  Gomez-Gomez L, Bauer Z, Boller T (2001) Both the extracellular leucine-rich repeat domain and the kinase activity of FSL2 are required for flagellin binding and signaling in Arabidopsis. Plant Cell 13(5):1155–1163CrossRef PubMed PubMedCentral
  Hann DR, Rathjen JP (2007) Early events in the pathogenicity of Pseudomonas syringae on Nicotiana benthamiana. Plant J Cell Mol Biol 49(4):607–618CrossRef
  Heese A, Hann DR, Gimenez-Ibanez S, Jones AM, He K, Li J, Schroeder JI, Peck SC, Rathjen JP (2007) The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc Natl Acad Sci USA 104(29):12217–12222CrossRef PubMed PubMedCentral
  Hu H, Xiong L, Yang Y (2005) Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection. Planta 222(1):107–117CrossRef PubMed
  Katagiri F, Tsuda K (2010) Understanding the plant immune system. Mol Plant-microb Interact MPMI 23(12):1531–1536CrossRef
  Kauffman HE, Reddy APK, Hsieh SPY, Merca SD (1973) An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep 57:537–541
  Kemmerling B, Schwedt A, Rodriguez P, Mazzotta S, Frank M, Qamar SA, Mengiste T, Betsuyaku S, Parker JE, Mussig C, Thomma BP, Albrecht C, de Vries SC, Hirt H, Nurnberger T (2007) The BRI1-associated kinase 1, BAK1, has a brassinolide-independent role in plant cell-death control. Curr Biol CB 17(13):1116–1122CrossRef PubMed
  Kinoshita T, Cano-Delgado A, Seto H, Hiranuma S, Fujioka S, Yoshida S, Chory J (2005) Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature 433(7022):167–171CrossRef PubMed
  Li J, Wen J, Lease KA, Doke JT, Tax FE, Walker JC (2002) BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell 110(2):213–222CrossRef PubMed
  Li D, Wang L, Wang M, Xu YY, Luo W, Liu YJ, Xu ZH, Li J, Chong K (2009) Engineering OsBAK1 gene as a molecular tool to improve rice architecture for high yield. Plant Biotechnol J 7(8):791–806CrossRef PubMed
  Lin W, Li B, Lu D, Chen S, Zhu N, He P, Shan L (2014) Tyrosine phosphorylation of protein kinase complex BAK1/BIK1 mediates Arabidopsis innate immunity. Proc Natl Acad Sci USA 111(9):3632–3637CrossRef PubMed PubMedCentral
  Lu D, Wu S, Gao X, Zhang Y, Shan L, He P (2010) A receptor-like cytoplasmic kinase, BIK1, associates with a flagellin receptor complex to initiate plant innate immunity. Proc Natl Acad Sci USA 107(1):496–501CrossRef PubMed PubMedCentral
  Mei C, Qi M, Sheng G, Yang Y (2006) Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection. Mol Plant-microb Interact MPMI 19(10):1127–1137CrossRef
  Nam KH, Li J (2002) BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell 110(2):203–212CrossRef PubMed
  Park HS, Ryu HY, Kim BH, Kim SY, Yoon IS, Nam KH (2011) A subset of OsSERK genes, including OsBAK1, affects normal growth and leaf development of rice. Mol Cells 32(6):561–569CrossRef PubMed PubMedCentral
  Peck SC, Nuhse TS, Hess D, Iglesias A, Meins F, Boller T (2001) Directed proteomics identifies a plant-specific protein rapidly phosphorylated in response to bacterial and fungal elicitors. Plant Cell 13(6):1467–1475CrossRef PubMed PubMedCentral
  Robatzek S, Bittel P, Chinchilla D, Kochner P, Felix G, Shiu SH, Boller T (2007) Molecular identification and characterization of the tomato flagellin receptor LeFLS2, an orthologue of Arabidopsis FLS2 exhibiting characteristically different perception specificities. Plant Mol Biol 64(5):539–547CrossRef PubMed
  Roux M, Schwessinger B, Albrecht C, Chinchilla D, Jones A, Holton N, Malinovsky FG, Tor M, de Vries S, Zipfel C (2011) The Arabidopsis leucine-rich repeat receptor-like kinases BAK1/SERK3 and BKK1/SERK4 are required for innate immunity to hemibiotrophic and biotrophic pathogens. Plant Cell 23(6):2440–2455CrossRef PubMed PubMedCentral
  Sasabe M, Takeuchi K, Kamoun S, Ichinose Y, Govers F, Toyoda K, Shiraishi T, Yamada T (2000) Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture. Eur J Biochem/FEBS 267(16):5005–5013CrossRef
  Shan L, He P, Li J, Heese A, Peck SC, Nurnberger T, Martin GB, Sheen J (2008) Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host Microbe 4(1):17–27CrossRef PubMed PubMedCentral
  Takai R, Isogai A, Takayama S, Che FS (2008) Analysis of flagellin perception mediated by flg22 receptor OsFLS2 in rice. Mol Plant-microb Interact MPMI 21(12):1635–1642CrossRef
  Trda L, Fernandez O, Boutrot F, Heloir MC, Kelloniemi J, Daire X, Adrian M, Clement C, Zipfel C, Dorey S, Poinssot B (2014) The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria. New Phytol 201(4):1371–1384CrossRef PubMed
  Wang X, Goshe MB, Soderblom EJ, Phinney BS, Kuchar JA, Li J, Asami T, Yoshida S, Huber SC, Clouse SD (2005) Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase. Plant Cell 17(6):1685–1703CrossRef PubMed PubMedCentral
  Zipfel C (2008) Pattern-recognition receptors in plant innate immunity. Curr Opin Immunol 20(1):10–16CrossRef PubMed
  
• 作者单位：Hualan Liao (1)
  Xiaorong Xiao (1)
  Xiuqiong Li (1)
  Yan Chen (2)
  Xiumei Fu (1)
  Daozhe Lin (1)
  Xiaolei Niu (1)
  Yinhua Chen (1)
  Chaozu He (1)
  
  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Hainan University, Haikou, 570228, People’s Republic of China
  2. Beijing University of Agriculture, Beijing, 102206, People’s Republic of China
  
• 刊物主题：Plant Sciences; Cell Biology; Plant Biochemistry; Biotechnology; Agriculture;
• 出版者：Springer Japan
• ISSN：1863-5474

文摘

OsBAK1 gene belongs to a receptor like kinase gene family in rice and shares a highly conserved gene structure and sequence homology with Arabidopsis thaliana BAK1 gene. To investigate the role of OsBAK1 in rice immunity, the full-length cDNA of OsBAK1 was isolated by RT-PCR and the transgenic rice lines (over expression and RNA-interference lines) were generated using Agrobacterium-mediated transformation. The expression level of OsBAK1 was determined by q-PCR in overexpression and RNAi transgenic rice lines. Based on quantitative polymerase chain reaction (q-PCR) results, two overexpression lines and two RNAi lines were evaluated in bioassays for resistance to Xanthomonas oryzae pv. oryzae PXO99, the causal agent of rice bacterial blight disease. Pathogenicity bioassays showed overexpression OsBAK1 lines exhibited resistance to blight disease whereas OsBAK1 RNAi lines promoted susceptibility. Besides, OsBAK1 can complement the function of AtBAK1 in Arabidopsis bak1 protoplast, activating FRK1 expression, a marker gene in PTI signaling pathway, after treatment by flg22. Furthermore, the transcriptional level of OsBAK1 was induced significantly in rice by defense signaling molecules such as salicylic acid, jasmonic acid, and PXO99 inoculation. Our results illustrated OsBAK1 positively regulates plant defense against rice bacterium pathogen Xanthomonas oryzae pv. oryzae PXO99.