Reporter Cell Lines to Monitor Steroid and Antisteroid Potential of Environmental Samples

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• 作者：Patrick Balaguer ; H¨¦l¨¨ne Fenet ; Virginie Georget ; Franck Comunale ; B¨¦atrice T¨¦rouanne ; Rodolphe Gilbin ; Elena Gomez ; Anne-Marie Boussioux ; Charles Sultan and Michel Pons ; et al.
• 关键词：xenohormones ; reporter cell lines ; steroid receptors ; wastewater effluent ; sediments
• 刊名：Ecotoxicology
• 出版年：2000
• 出版时间：2000
• 年：2000
• 卷：9
• 期：1/2
• 页码：105-114
• 全文大小：113 KB

文摘

In order to monitor the (anti)steroid activity of environmental samples, we established stable cell lines expressing luciferase under the control of estrogens, androgens, progestives and glucocorticoids. The breast cancer MCF-7 cells which express the estrogen receptor (ER), the glucocorticoid receptor (GR) and the progesterone receptor (PR) were transfected by an estrogen (ERE-Glob-Luc) or a glucocorticoid/progestin/androgen (MMTV-Luc) regulated luciferase plasmid in order to enable the detection of compounds which bind both ER, PR and GR (MELN and MMLN cells). Human prostatic cells PC3 were stably transfected by both an androgen receptor gene and the MMTV-luciferase plasmid (PALM cells). These three cellular models were validated as tools to check the estrogenic, progestive, glucocorticoid and androgenic activities using several potential xenohormones and environmental samples. As these environmental samples were fractionated after solid phase extraction to isolate active compounds, we used these cellular models to monitor the different fractions. In the estrogenic model mid-polar fractions of environmental samples were found active while in the androgenic model, the same fractions had antagonist activity.