文摘
Sterols in the seeds of wild Finnish blueberry (Vaccinium myrtillus) and lingonberry (Vaccinium vitis-idaea) were analyzed as TMS derivatives by gas chromatography-mass spectrometry. Free and esterified sterols constituted 0.7% and 0.3% of the seed oil of V. myrtillus, respectively, whereas in the seed oil of V. vitis-idaea the sterols in the two fractions were equally represented at 0.5-0.6%. Sitosterol (85% in V. myrtillus and 80% in V. vitis-idaea) and campesterol (7% in V. myrtillus and 6% in V. vitis-idaea) were the dominant free sterols. In comparison to free sterols, steryl esters were found to have considerably lower proportions of sitosterol (40-60%) and campesterol (3-5%), accompanied by higher levels of intermediates in the biosynthetic cascade. Although both species contained the same major sterols, they differed in their relative abundances of individual compounds. Specifically, blueberry contained a higher proportion of sitosterol and campesterol and a lower proportion of isofucosterol and cycloartenol. Comparison of berries collected from northern and southern Finland showed that growth conditions have little effect on the sterol compositions in the berries. To our knowledge this is the first report on the content and composition of phytosterols in Vaccinium sp. The results provide important information on the chemical compositions, nutritional properties, and possible chemotaxonomical characteristics of the two species.