Multi-color reflectance imaging of middle ear pathology in vivo

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• 作者：Tulio A. Valdez (1)
  Nicolas Spegazzini (2)
  Rishikesh Pandey (2)
  Kaitlyn Longo (3)
  Christopher Grindle (1)
  Donald Peterson (3)
  Ishan Barman (4)
  
  1. Department of Pediatric Otolaryngology ; Connecticut Children鈥檚 Medical Center ; Hartford ; CT ; 06106 ; USA
  2. G. R. Harrison Spectroscopy Laboratory ; Massachusetts Institute of Technology ; Cambridge ; MA ; 02139 ; USA
  3. Biodynamics Laboratory ; University of Connecticut Health Center ; Farmington ; CT ; 06030 ; USA
  4. Department of Mechanical Engineering and Oncology ; Johns Hopkins University ; Baltimore ; MD ; 21218 ; USA
  
• 关键词：Otoscopy ; Acute otitis media ; Reflectance ; Autofluorescence ; Imaging ; Medical device
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：407
• 期：12
• 页码：3277-3283
• 全文大小：2,124 KB
• 参考文献：1. Blomgren, K, Pitkaranta, A (2005) Int J Pediatr Otorhinolaryngol 69: pp. 295-299 class="external" href="http://dx.doi.org/10.1016/j.ijporl.2004.09.012" target="_blank" title="It opens in new window">CrossRef
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Otoscopic examination using white-light illumination has remained virtually unchanged for well over a century. However, the limited contrast of white-light otoscopy constrains the ability to make accurate assessment of middle ear pathology and is subject to significant observer variability. Here, we employ a modified otoscope with multi-color imaging capabilities for superior characterization of the middle ear constituents in vivo and for enhanced diagnosis of acute otitis media and cholesteatoma. In this pilot study, five patients undergoing surgery for tympanostomy tube placement and congenital cholesteatoma excision were imaged using the custom-designed multi-color video-rate reflectance imaging system. We show that the multi-color imaging approach offers an increase in image contrast, thereby enabling clear visualization of the middle ear constituents, especially of the tympanic membrane vascularity. Differential absorption at the multiple wavelengths provides a measure of biochemical and morphological information, and the rapid acquisition and analysis of these images aids in objective evaluation of the middle ear pathology. Our pilot study shows the potential of using label-free narrow-band reflectance imaging to differentiate middle ear pathological conditions from normal middle ear. This technique can aid in obtaining objective and reproducible diagnoses as well as provide assistance in guiding excisional procedures.