A rare fraction of human hematopoietic stem cells with large telomeres

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• 作者：Marta García-Escarp ; Vanessa Martinez-Mu?oz ; Jordi Barquinero ; Irene Sales-Pardo ; Joan Carles Domingo ; Pedro Marin and Jordi Petriz
• 关键词：Telomere length ; Stem cells ; CD34 ; Flow ; FISH ; Flow cytometry ; Human
• 刊名：Cell and Tissue Research
• 出版年：2005
• 出版时间：March 2005
• 年：2005
• 卷：319
• 期：3
• 页码：405-412
• 全文大小：264 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

The lack of specific markers for stem cells makes the physical identification of this compartment difficult. Hematopoietic stem cells differ in their repopulating and self-renewal potential. Our study shows that multiple classes of human hematopoietic CD34+ greatly differ in telomere length. Flow-cytometry-based fluorescent in situ hybridization and confocal microscopy of CD34+ cells has revealed remarkable telomere length heterogeneity, with a hybridization pattern consistent with different classes of human hematopoietic progenitor cells. These results also point to the existence of a significant clonal heterogeneity among primitive hematopoietic cells and provide the first evidence of a rare fraction of CD34+ cells with large telomeres in humans.