The efficacy of a selamectin (Stronghold?) spot on treatment in the prevention of Bartonella henselae transmission by Ctenocephalides felis in cats, using a new hig

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• 作者：Emilie Bouhsira ; Michel Franc ; Emmanuel Lienard ; Corinne Bouillin…
• 关键词：Bartonellosis ; Bartonella henselae ; Selamectin ; New challenge model ; Fleas ; Flea control
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：114
• 期：3
• 页码：1045-1050
• 全文大小：296 KB
• 参考文献：1. Abbott RC, Chomel BB, Kasten RW, Floyd-Hawkins KA, Kikuchi Y, Koehler JE, Pedersen NC (1997) Experimental and natural infection with / Bartonella henselae in domestic cats. Comp Immunol Microbiol Infect Dis 20:41-1 CrossRef
  2. Beugnet F, Franc M (2012) Insecticide and acaricide molecules and/or combinations to prevent pet infestation by ectoparasites. Trends Parasitol 28:267-79 CrossRef
  3. Bouhsira E, Ferrandez Y, Liu M, Franc M, Boulouis HJ, Biville F (2013a) / Ctenocephalides felis an in vitro potential vector for five / Bartonella species. Comp Immunol Microbiol Infect Dis 36:105-11 CrossRef
  4. Bouhsira E, Franc M, Boulouis HJ, Jacquiet P, Raymond-Letron I, Liénard E (2013b) Assessment of persistence of / Bartonella henselae in / Ctenocephalides felis. Appl Environ Microbiol 79:7439-444 CrossRef
  5. Boulouis HJ, Chang CC, Henn JB, Kasten RW, Chomel BB (2005) Factors associated with the rapid emergence of zoonotic / Bartonella infections. Vet Res 36:383-10 CrossRef
  6. Bradbury CA, Lappin MR (2010) Evaluation of topical application of 10% imidacloprid-1% moxidectin to prevent / Bartonella henselae transmission from cat fleas. J Am Vet Med Assoc 236:869-73 CrossRef
  7. Breitschwerdt EB (2008) Feline bartonellosis and cat scratch disease. Vet Immunol Immunopathol 123:167-71 CrossRef
  8. Breitschwerdt EB, Kordick DL (1995) Bartonellosis. J Am Vet Med Assoc 206:1928-931
  9. Breitschwerdt EB, Kordick DL (2000) / Bartonella infection in animals: carriership, reservoir potential, pathogenicity, and zoonotic potential for human infection. Clin Microbiol Rev 13:428-38 CrossRef
  10. Chomel BB, Kasten RW (2010) Bartonellosis, an increasingly recognized zoonosis. J Appl Microbiol 109:743-50 CrossRef
  11. Chomel BB, Abbott RC, Kasten RW, Floyd-Hawkins KA, Kass PH, Glaser CA, Pedersen NC, Koehler JE (1995) / Bartonella henselae prevalence in domestic cats in California: risk factors and association between bacteraemia and antibody titers. J Clin Microbiol 33:2445-450
  12. Chomel BB, Kasten RW, Floyd-Hawkins K, Chi B, Yamamoto K, Roberts-Wilson J, Gurfield AN, Abbott RC, Pedersen NC, Koehler JE (1996) Experimental transmission of / Bartonella henselae by the cat flea. J Clin Microbiol 34:1952-956
  13. Chomel BB, Wey AC, Kasten RW, Stacy BA, Labelle P (2003) Fatal case of endocarditis associated with / Bartonella henselae type I infection in a domestic cat. J Clin Microbiol 41:5337-339 CrossRef
  14. Chomel BB, Boulouis HJ, Breitschwerdt EB (2004) Cat scratch disease and other zoonotic Bartonella infections. J Am Vet Med Assoc 224:1270-279 CrossRef
  15. Dryden MW1, Smith V, Payne PA, McTier TL. (2005) Comparative speed of kill of selamectin, imidacloprid, and fipronil-(S)-methoprene spot-on formulations against fleas on cats. Vet Ther. 2005 Fall;6(3):228-6
  16. Eisen RJ, Gage KL (2012) Transmission of flea-borne zoonotic agents. Annu Rev Entomol 57:61-2 CrossRef
  17. Finkelstein JL, Brown TP, O’Reilly KL, Wedincamp J Jr, Foil LD (2002) Studies on the growth of / Bartonella henselae in the cat flea (Siphonaptera: Pulicidae). J Med Entomol 39:915-19 CrossRef
  18. Foil L, Andress E, Freeland RL, Roy AF, Rutledge R, Triche PC, O’Reilly KL (1998) Experimental infection of domestic cats with / Bartonella henselae by inoculation of / Ctenocephalides felis (
• 作者单位：Emilie Bouhsira (1)
  Michel Franc (1)
  Emmanuel Lienard (1)
  Corinne Bouillin (2)
  Christelle Gandoin (2)
  Thomas Geurden (3)
  Csilla Becskei (3)
  Philippe Jacquiet (1)
  Anne Thomas (3)
  Henri Jean Boulouis (2)
  
  1. INP Ecole Nationale Vétérinaire, Toulouse, France
  2. UMR BIPAR, ENVA, Anses, Maisons Alfort, 94700, France
  3. Zoetis, Mercuriusstraat 20, B 1930, Zaventem, Belgium
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Bartonella henselae is the causative agent of cat scratch disease in humans, which is recognized as an emerging zoonotic disease. Ctenocephalides felis is the main vector, and transmission of B. henselae infection between cats and humans occurs mainly through infected flea feces. Control of feline infestation with this arthropod vector therefore provides an important strategy for the prevention of infection of both humans and cats. In the present study, a new challenge model is used to evaluate the efficacy of selamectin (Stronghold? spot on) in the prevention of B. henselae transmission by C. felis. In this new challenge model, domestic cats were infected by direct application of B. henselae-positive fleas. The fleas used for infestation were infected by feeding on blood that contained in vitro-cultured B. henselae. The direct application of the fleas to the animals and the use of different B. henselae strains ensured a high and consistent challenge. Two groups of six cats were randomly allocated on pre-treatment flea counts to either control (untreated cats) or the selamectin-treated group with one pipette per cat according to the label instruction. Stronghold (selamectin 6?% spot on solution) was administered on days 0 and 32. On days 3, 10, 19, 25, and 31, each cat was infested by direct application of 20 fleas that fed on blood inoculated with B. henselae. Polymerase chain reaction (PCR) on pooled fleas confirmed that the fleas were infected. Blood samples were collected from each cat on days ? (prior to flea infestation and treatment), 9, 17, 24, 30, 37, and 44 and assayed for B. henselae antibodies using an indirect immunofluorescence (IFA), for the presence of bacteria by bacterial culture and for B. henselae DNA presence by PCR. Cats were also assessed on a daily basis for general health. There were no abnormal health observations during the study and none of the animals required concomitant treatment. None of the cats displayed any clinical signs of bartonellosis during the study. In the untreated group, all cats became bacteremic within 17 to 44?days. None of the selamectin-treated cats became positive during the study. It was concluded that Stronghold? spot on administered to cats was efficacious in the prevention of the transmission of B. henselae by fleas to cats in a high-challenge model.