Molecular survey of arthropod-borne pathogens in sheep keds (Melophagus ovinus), Central Europe
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- 作者：Ivo Rudolf ; Lenka Betášová ; Vlastimil Bischof ; Kristýna Venclíková…
- 关键词：Melophagus ovinus ; Bartonella melophagi ; Rickettsiae ; Arboviruses ; Borrelia burgdorferi ; Babesia spp.
- 刊名：Parasitology Research
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：115
- 期：10
- 页码：3679-3682
- 全文大小：310 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biomedicine
Medical Microbiology
Microbiology
Immunology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-1955
- 卷排序：115
文摘
In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phagocytophilum, “Candidatus Neoehrlichia mikurensis,” and Babesia spp. All ked pools were positive for Bartonella DNA. The sequencing of the amplified fragments of the gltA and 16S-23S rRNA demonstrated a 100 % homology with Bartonella melophagi previously isolated from a sheep ked and from human blood in the USA. The identification of B. melophagi in sheep keds in Central Europe highlights needs extending a list of hematophagous arthropods beyond ticks and mosquitoes for a search of emerging arthropod-borne pathogens.