In vitro induction, screening and detection of high essential oil yielding somaclones in turmeric (Curcuma longa L.)

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• 作者：Basudeba Kar (1)
  Ananya Kuanar (1)
  Sikha Singh (1)
  Sujata Mohanty (1)
  Raj Kumar Joshi (1)
  Enketeswara Subudhi (1)
  Sanghamitra Nayak (1)
  
• 关键词：In vitro selection ; Oil yield ; Somaclone ; Turmeric
• 刊名：Plant Growth Regulation
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：72
• 期：1
• 页码：59-66
• 全文大小：351 KB
• 作者单位：Basudeba Kar (1)
  Ananya Kuanar (1)
  Sikha Singh (1)
  Sujata Mohanty (1)
  Raj Kumar Joshi (1)
  Enketeswara Subudhi (1)
  Sanghamitra Nayak (1)
  
  1. Center of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, 751003, India
  
• ISSN：1573-5087

文摘

Leaf of turmeric contains an essential oil used extensively in perfumery, pharmaceuticals and aromatherapy. Five somaclones were induced in turmeric on MS media with varying amounts of plant growth regulators. All somaclones were subsequently transferred to the field. Essential oil was extracted from leaves of in vitro and ex vitro grown plants and subjected to quantitative and qualitative evaluation. A positive correlation was established between the leaf oil content and oil constituent of in vitro grown and field transferred somaclones. Somaclones (C2, C4, C5) containing 0.16-.18?% oil in vitro retained normal oil content (0.48-.5?%) in the field. Similarly in vitro grown somaclones C3 and C7 with 0.36 and 0.25?% oil content retained proportionately increased oil yields of 1?% and 0.76 under ex vitro condition. GC–MS analysis of the oil revealed similar spectrum of constituents both among in vitro and ex vitro grown plants with alpha-phellandrene as major one. Thus the novel method of in vitro screening could be applied for rapid identification of high essential oil yielding turmeric genotypes thereby reducing labour, cost and time required in conventional ex vitro screening of somaclones.