Optimization of Dilute Acid Pretreatment and Enzymatic Hydrolysis of Phalaris aquatica L. Lignocellulosic Biomass in Batch and Fed-Batch Processes

详细信息    查看全文

• 作者：Anna Karapatsia ; Ioannis Pappas ; Giannis Penloglou…
• 关键词：Acid pretreatment ; Enzymatic hydrolysis ; Fed ; batch operation ; Biomass particle size ; Lignocellulose ; Taguchi DOE
• 刊名：BioEnergy Research
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：10
• 期：1
• 页码：225-236
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Plant Ecology; Wood Science & Technology;
• 出版者：Springer US
• ISSN：1939-1242
• 卷排序：10

文摘

Phalaris aquatica L., a rich in holocellulose (69.80 %) and deficient in lignin (6.70 %) herbaceous, perennial grass species, was utilized in a two-step (biomass pretreatment-enzymatic hydrolysis) saccharification process for sugars recovery. The Taguchi methodology was employed to determine the dilute acid pretreatment and enzymatic hydrolysis conditions that optimized hemicellulose conversion (75.04 %), minimized the production of inhibitory compounds (1.41 g/L), and maximized the cellulose to glucose yield (69.69 %) of mixed particulate biomass (particles <1000 μm) under batch conditions. The effect of biomass particle size on saccharification process efficiency was also investigated. It was found that small-size biomass particles (53–106 μm) resulted in maximum hemicellulose conversion (81.12 %) and cellulose to glucose yield (93.24 %). The determined optimal conditions were then applied to a combined batch pretreatment process followed by a fed-batch enzymatic hydrolysis process that maximized glucose concentration (62.24 g/L) and yield (92.48 %). The overall efficiency of the saccharification process was 88.13 %.