Fumaric Acid Production from Alkali-Pretreated Corncob by Fed-Batch Simultaneous Saccharification and Fermentation Combined with Separated Hydrolysis and Fermentation at High Solids Loading
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- 作者：Xin Li ; Jin Zhou ; Shuiping Ouyang ; Jia Ouyang…
- 关键词：Fumaric acid ; Rhizopus oryzae ; Alkali ; pretreated corncob ; High solids loading ; Fed ; batch simultaneous saccharification and fermentation
- 刊名：Applied Biochemistry and Biotechnology
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：181
- 期：2
- 页码：573-583
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Biotechnology; Biochemistry, general;
- 出版者：Springer US
- ISSN：1559-0291
- 卷排序：181
文摘
Production of fumaric acid from alkali-pretreated corncob (APC) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (SHF) and fed-batch simultaneous saccharification and fermentation (SSF) by Rhizopus oryzae. Four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. The highest concentration of 41.32 g/L fumaric acid was obtained from 20 % (w/v) APC at 38 °C in the combined SHF and fed-batch SSF process, compared with 19.13 g/L fumaric acid in batch SSF alone. The results indicated that a combination of SHF and fed-batch SSF significantly improved production of fumaric acid from lignocellulose by R. oryzae than that achieved with batch SSF at high solids loading.