Negative regulation of the RTBV promoter by designed zinc finger proteins

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• 作者：M. Isabel Ordiz ; Laurent Magnenat ; Carlos F. Barbas and Roger N. Beachy
• 关键词：Gene regulation ; Repression ; RTBV promoter ; Transcription factors ; Synthetic zinc fingers
• 刊名：Plant Molecular Biology
• 出版年：2010
• 出版时间：April, 2010
• 年：2010
• 卷：72
• 期：6
• 页码：621-630
• 全文大小：538.3 KB

文摘

The symptoms of rice tungro disease are caused by infection by a DNA-containing virus, rice tungro bacilliform virus (RTBV). To reduce expression of the RTBV promoter, and to ultimately reduce virus replication, we tested three synthetic zinc finger protein transcription factors (ZF-TFs), each comprised of six finger domains, designed to bind to sequences between −58 and +50 of the promoter. Two of these ZF-TFs reduced expression from the promoter in transient assays and in transgenic Arabidopsis thaliana plants. One of the ZF-TFs had significant effects on plant regeneration, apparently as a consequence of binding to multiple sites in the A. thaliana genome. Expression from the RTBV promoter was reduced by ~45 % in transient assays and was reduced by up to 80 % in transgenic plants. Co-expression of two different ZF-TFs did not further reduce expression of the promoter. These experiments suggest that ZF-TFs may be used to reduce replication of RTBV and thereby offer a potential method for control of an important crop disease.