文摘
Near infrared-reflectance spectroscopy (NIRS) is widely used as an efficient and non-destructive method for seed quality analysis in oilseed rape (Brassica napus L.). Normally, calibration equations are developed for seed samples sizes of 3–4 g. However, during the breeding process much less than 3 g of samples are frequently only available for analysis. The objective of the present study was to analyse whether calibration equations developed for standard sample sizes can be used to predict the oil, protein, moisture, glucosinolate and fatty acid content of sample sizes ranging from about 450 to 5 mg (single seeds). Special adapters were made for the standard ring cups to hold the smaller samples during NIRS scanning. Close Spearman rank correlations (r S