Neospora caninum infection during early pregnancy in cattle: how the isolate influences infection dynamics, clinical outcome and peripheral and local immune responses

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• 作者：Javier Regidor-Cerrillo (9)
  David Arranz-Solís (9)
  Julio Benavides (10)
  Mercedes Gómez-Bautista (9)
  José Antonio Castro-Hermida (11)
  Mercedes Mezo (11)
  Valentín Pérez (10)
  Luis Miguel Ortega-Mora (9)
  Marta González-Warleta (11)
  
• 刊名：Veterinary Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：45
• 期：1
• 全文大小：524 KB
• 作者单位：Javier Regidor-Cerrillo (9)
  David Arranz-Solís (9)
  Julio Benavides (10)
  Mercedes Gómez-Bautista (9)
  José Antonio Castro-Hermida (11)
  Mercedes Mezo (11)
  Valentín Pérez (10)
  Luis Miguel Ortega-Mora (9)
  Marta González-Warleta (11)
  
  9. Animal Health Department, SALUVET, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain
  10. Livestock Health and Production Institute (ULE-CSIC), Grulleros, 24346, León, Spain
  11. Laboratory of Parasitology, Agricultural Research Centre of Mabegondo (CIAM-INGACAL), Xunta de Galicia, Carretera AC-542 de Betanzos a Mesón do Vento, km 7, 15318, Abegondo, A Coru?a, Spain
  
• ISSN：1297-9716

文摘

This work studies the influence of Neospora caninum intra-species diversity on abortion outcome, infection dynamics in terms of parasite dissemination and peripheral-local immune responses in pregnant cattle. Animals were intravenously inoculated at day 70 of pregnancy with 107 tachyzoites of two isolates showing marked differences in virulence in vitro and in pregnant mouse models: Nc-Spain7, a high virulence isolate, and Nc-Spain8, a low-to-moderate virulence isolate. After inoculation, pregnancy was monitored, and dams were culled when foetal death was detected. Foetal mortality occurred in all infected heifers between days 24 and 49 post-infection (pi), however, it was detected sooner in Nc-Spain7-infected animals (median day--4) than those inoculated with Nc-Spain8 (median day--1) with a trend towards significance (P-lt;-.11). Similar histological lesions were observed in placentomes and in most of the foetuses from the two infected groups. However, parasites were more frequently detected in the placenta and foetuses by PCR and in the foetal brain by immunohistochemistry in Nc-Spain7-infected animals. Specific antibodies were detected starting at day 13 post-infection in all infected cattle, with higher IgG levels in Nc-Spain7-infected group. IFN-γ and IL-4 profiles also varied between infected groups in PBMC stimulation assays. Infected animals showed significant increases in their cytokine mRNA levels (IFN-γ, IL-4, IL-10, IL-12p40 and TNF-α) in the caruncle at time of foetal death. Differences between the infected groups were also observed for cytokine profiles. These results demonstrate the influence of the N. caninum isolate on foetal death outcome, infection dynamics and immune responses in cattle.