Effects of the utilization of homeopathic elements in commercial diluent on swine sperm viability

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• 作者：Francisco Rafael Martins Soto (1) r> Erlete Rosalina Vuaden (2) r> Cideli de Paula Coelho (3) r> Leoni Villano Bonamin (4) r> Sérgio Santos de Azevedo (5) r> Nilson Roberti Benites (3) r> Flavia Regina Oliveira de Barros (3) r> Marcelo Demarchi Goissis (3) r> Mayra Elena Ortiz D’ávila Assump??o (3) r> José Ant?nio Visintin (3) r> Mariana Groke Marques (6) r>
• 关键词：Homeopathy ; Swine semen ; Sperm viability
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：47
• 期：3
• 页码：205-209
• 全文大小：169KB
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• 作者单位：Francisco Rafael Martins Soto (1) r> Erlete Rosalina Vuaden (2) r> Cideli de Paula Coelho (3) r> Leoni Villano Bonamin (4) r> Sérgio Santos de Azevedo (5) r> Nilson Roberti Benites (3) r> Flavia Regina Oliveira de Barros (3) r> Marcelo Demarchi Goissis (3) r> Mayra Elena Ortiz D’ávila Assump??o (3) r> José Ant?nio Visintin (3) r> Mariana Groke Marques (6) r>r>1. Center for Sanitary Surveillance and Zoonoses Control “Tereza Rodrigues de Camargo- Estrada da Vargem do Salto- Km 4,5- Bairro dos Paes, 18150-000, Ibiúna, S?o Paulo, Brazil r> 2. M Cassab- Animal Technology, Avenida das Na??es Unidas, 20.882, 04795-000, S?o Paulo, S?o Paulo, Brazil r> 3. Department of Animal Reproduction, College of Veterinary Medicine and Animal Sciences, University of Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-900, S?o Paulo, S?o Paulo, Brazil r> 4. Faculty of Veterinary Medicine, Paulista University, Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-900, S?o Paulo, S?o Paulo, Brazil r> 5. Academic Unity of Veterinary Medicine, University of Campina Grande, Avenida Universitária, s/n, Bairro Santa Cecília, Caixa Postal 64, 58700-970, Patos, Paraíba, Brazil r> 6. EMBRAPA Swine and Poultry Research Center, BR 153, km 110, Tamandua, 89700-000, Concordia, Santa Catarina, Brazil r>

文摘

It has been speculated that the homeopathic treatment of sperm cells in order to improve semen quality could be promising. However, few data is available and its use in spermatozoa requires investigation. It is well established that mitochondrial membrane potential is an important viability parameter of spermatozoa and it is intimately related to reproductive efficiency. In this manner, new technologies in order to improve the activity of sperm cells and, finally, the fecundity of swine herds are of extremely importance. Due to the lack of knowledge of homeopathic treatment effect on spermatozoa, the aim of the present study was to verify the effect of three different homeopathic treatments on viability of boar sperm cells. Three homeopathic treatments composed by Pulsatila CH6, Pulsatila and Avena CH6, Avena CH6 and one control treatment (sucrose) were added to diluted boar semen, which were cooled for 24 or 48?h. Interestingly, no positive effect of homeopathic treatments was observed over semen viability. However, it was demonstrated that the 24?h of cooling storage provided more viable sperm cells when compared to the 48-h period. This effect of storage period on sperm viability was assessed by intact plasmatic membrane, intact acrosome and mitochondrial membrane potential evaluation.