Comparative study of endophytic and endophytic diazotrophic bacterial communities across rice landraces grown in the highlands of northern Thailand

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• 作者：Chakrapong Rangjaroen (1)
  Benjavan Rerkasem (2)
  Neung Teaumroong (3)
  Rungroch Sungthong (4)
  Saisamorn Lumyong (1)
  
• 关键词：Bacterial community ; Bacterial endophytes ; 16S rRNA gene ; nifH gene ; Cluster analysis ; Principal component analysis
• 刊名：Archives of Microbiology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：196
• 期：1
• 页码：35-49
• 全文大小：1,110 KB
• 作者单位：Chakrapong Rangjaroen (1)
  Benjavan Rerkasem (2)
  Neung Teaumroong (3)
  Rungroch Sungthong (4)
  Saisamorn Lumyong (1)
  
  1. Microbiology Division, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand
  2. Faculty of Agriculture, Lanna Rice Research and Cultural Centre, Chiang Mai University, Chiang Mai, 50200, Thailand
  3. Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, Nakhonratchasima, 30000, Thailand
  4. Departamento de Agroquímica y Conservación de Suelos, Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS-CSIC), Seville, 41080, Spain
  
• ISSN：1432-072X

文摘

Communities of bacterial endophytes within the rice landraces cultivated in the highlands of northern Thailand were studied using fingerprinting data of 16S rRNA and nifH genes profiling by polymerase chain reaction–denaturing gradient gel electrophoresis. The bacterial communities-richness, diversity index, evenness, and stability were varied depending on the plant tissues, stages of growth, and rice cultivars. These indices for the endophytic diazotrophic bacteria within the landrace rice Bue Wah Bo were significantly the lowest. The endophytic bacteria revealed greater diversity by cluster analysis with seven clusters compared to the endophytic diazotrophic bacteria (three clusters). Principal component analysis suggested that the endophytic bacteria showed that the community structures across the rice landraces had a higher stability than those of the endophytic diazotrophic bacteria. Uncultured bacteria were found dominantly in both bacterial communities, while higher generic varieties were observed in the endophytic diazotrophic bacterial community. These differences in bacterial communities might be influenced either by genetic variation in the rice landraces or the rice cultivation system, where the nitrogen input affects the endophytic diazotrophic bacterial community.