Effect of benzo[a]pyrene on detoxification and the activity of antioxidant enzymes of marine microalgae

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• 作者：Chen Shen ; Jingjing Miao ; Yun Li ; Luqing Pan
• 关键词：benzo[a]pyrene ; marine microalga ; detoxifying enzyme ; antioxidant enzyme ; lipid peroxidation
• 刊名：Journal of Ocean University of China
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：15
• 期：2
• 页码：303-310
• 全文大小：728 KB
• 参考文献：Bebianno, M. J., and Barreira, L. A., 2009. Polycyclic aromatic hydrocarbons concentrations and biomarker responses in the clam Ruditapes decussatus transplanted in the Ria Formosa lagoon. Ecotoxicology and Environmental Safety, 72(7): 1849–1860, DOI: 10.1016/j.ecoenv.2009.03.016.CrossRef
  Bo, J., Gopalakrishnan, S., Chen, F. Y., and Wang, K. J., 2014. Benzo[a]pyrene modulates the biotransformation, DNA damage and cortisol level of red sea bream challenged with lipopolysaccharide. Marine Pollution Bulletin, 85(2): 463–470, DOI: 10.1016/j.marpolbul.2014.05.023.CrossRef
  Bradford, M. A., 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 240–254, DOI: 10.1016/0003-2697(76)90527-3.CrossRef
  Brammell, B. F., Price, D. J., Birge, W. J., Harmel-Laws, E. M., Hitron, J. A., and Elskus, A. A., 2010. Differential sensitivity of CYP1A to 3,3’,4’,4-tetrachlorobip henyl and benzo(a) pyrene in two Lepomis species. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1): 42–50, DOI: 10.1016/j.cbpc.2010.02.008.
  Chen, L. Z., Zhou, L., Liu, Y. D., Deng, S. Q., Wu, H., and Wang, G. H., 2012. Toxicological effects of nanometer titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii. Ecotoxicology and Environmental Safety, 84: 155–162, DOI: 10.1016/j.ecoenv.2012.07.019.CrossRef
  Cima, F., Ferrari, G., Ferreira, N. G., Rocha, R. J., Serodio, J., Loureiro, S., and Calado, R., 2013. Preliminary evaluation of the toxic effects of the antifouling biocide Sea-Nine 211 in the soft coral Sarcophyton cf. glaucum (Octocorallia, Alcyonacea) based on PAM fluorometry and biomarkers. Marine Environmental Research, 83: 16–22, DOI: 10.1016/j.marenvres.2012.10.004.CrossRef
  Correa-Reyes, G., Viana, M. T., Marquez-Rocha, F. J., Licea, A. F., Ponce, E., and Vazquez-Duhalt, R., 2007. Nonylphenol algal bioaccumulation and its effect through the trophic chain. Chemosphere, 68: 662–670, DOI: 10.1016/j.chemosphere.2007.02.030.CrossRef
  da Silva Rocha, A. J., Gomes, V., Rocha Passos, M. J., Hasue, F. M., Alves Santos, T. C., Bicego, M. C., Taniguchi, S., and van Ngan, P., 2012. EROD activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene (BaP) concentrations. Environmental Toxicology and Pharmacology, 34(3): 995–1003, DOI: 10.1016/j.etap.2012.07.006.CrossRef
  David, R. M., Winter, M. J., and Chipman, J. K., 2009. Induction of DNA strand breaks by genotoxicants in the alga Chlamydomonas reinhardtii. Environmental Toxicology and Chemistry, 28(9): 1893–1900, DOI: 10.1897/08-349.1.CrossRef
  Gao, Q. T., and Tam, N. F., 2011. Growth, photosynthesis and antioxidant responses of two microalgal species, Chlorella vulgaris and Selenastrum capricornutum, to nonylphenol stress. Chemosphere, 82(3): 346–354, DOI: 10.1016/j.chemos phere.2010.10.010.CrossRef
  Gao, X. Y., Shi, X. R., Cui, Y. B., Li, M., Zhang, R. F., Qian, X., and Jiang, Y., 2011. Organic pollutants and ambient severity for the drinking water source of western Taihu Lake. Ecotoxicology, 20: 959–967, DOI: 10.1007/s10646-011-0681-6.CrossRef
  Habig, W. H., Pabst, M. J., and Jakoby, W. B., 1974. Glutathione S-Transferases the first enzymetic step in mercapturic acid formation. The Journal of Biological Chemistry, 249: 7130–7139.
  Hao, Z. B., 2004. Experiments in Plant Physiology. Harbin Institute of Technology Press, Harbin, 147pp (in Chinese).
  Jiang, J., 2010. The toxic effects of naphthalene on Scenedesmus obliquus and Microcystis aeruginosa. Master thesis. Northeast Normal University, Changchun (in Chinese).
  Lei, A. P., Wong, Y. S., and Tam, N. F., 2003. Pyrene-induced changes of glutathione-S-transferase activities in different microalgal species. Chemosphere, 50(3): 293–301, DOI: 10. 1016/S0045-6535(02)00499-X.CrossRef
  Li, M., Hu, C., Zhu, Q., Chen, L., Kong, Z., and Liu, Z., 2006. Copper and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in the microalga Pavlova viridis (Prymnesiophyceae). Chemosphere, 62(4): 565–572, DOI: 10. 1016/j.chemosphere.2005.06.029.CrossRef
  Li, M., Hu, C. G., Gao, X. Y., Xue, Y., Qian, X., Brown, M. T., and Cui, Y. B., 2009. Genotoxicity of organic pollutants in source of drinking water on microalga Euglena gracilis. Ecotoxicology, 18: 669–676, DOI: 10.1007/s10646-009-0343-0.CrossRef
  Lu, G. H., Chen, W., Li, Y., and Zhu, Z., 2011. Effects of PAHs on biotransformation enzymatic activities in fish. Chemcial Research in Chinese Universities, 27(3): 413–416.
  Mofeed, J., and Mosleh, Y. Y., 2013. Toxic responses and antioxidative enzymes activity of Scenedesmus obliquus exposed to fenhexamid and atrazine, alone and in mixture. Ecotoxicology and Environmental Safety, 95: 234–240, DOI: 10.1016/j.ecoenv.2013.05.023.CrossRef
  Mu, J. L., Wang, X. H., Jin, F., Wang, J. Y., and Hong, H. S., 2012. The role of cytochrome P4501A activity inhibition in three-to five-ringed polycyclic aromatic hydrocarbons embryotoxicity of marine medaka (Oryzias melastigma). Marine Pollution Bulletin, 64(7): 1445–1451, DOI: 10.1016/j.mar polbul.2012.04.007.CrossRef
  Pohl, R. J., and Fouts, J. R., 1980. A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions. Analytical Biochemistry, 107(1): 150–155, DOI: 10. 1016/0003-2697(80)90505-9.CrossRef
  Qian, H. F., Li, J. J., Pan, X. J., Sun, Z. Q., Ye, C. B., Jin, G. Q., and Fu, Z. W., 2010. Effects of Streptomycin on growth of algae Chlorella vulgaris amd Microcystis aeruginosa. Environmental Toxicology, 27(4): 229–237, DOI: 10.1002/tox. 20636.CrossRef
  Qiu, Y. W., Zhou, J. L., Maskaoui, K., Hong, H. S., and Wang, Z. D., 2004. Disrtibution of polycyclic aromatic hydrocarbons in water and sediments from DaYa Bay and their ecological hazard assessment. Journal of Tropical Oceanography, 23(4): 72–80, DOI: 10.3969/j.issn.1009-5470.2004.04.010 (in Chinese).
  Ren, X., Pan, L., and Wang, L., 2014. Toxic effects upon exposure to benzo[a]pyrene in juvenile white shrimp Litopenaeus vannamei. Environmental Toxicology and Pharmacology, 39(1): 194–207, DOI: 10.1016/j.etap.2014.08.006.CrossRef
  Sáenz, M. E., Di Marzio, W. D., and Alberdi, J. L., 2012. Assessment of Cyfluthrin commercial formulation on growth, photosynthesis and catalase activity of green algae. Pesticide Biochemistry and Physiology, 104: 50–57, DOI: 10.1016/j.pestbp.2012.07.001.CrossRef
  Shen, C., Li, Y., and Pan, L. Q., 2012. Effects of benzo[a]pyrene on cell growth and characteristics in marine microalgae. Marine Environmental Science, 31(4): 510–514.
  Silva, C., Oliveira, C., Gravato, C., and Almeida, J. R., 2013. Behaviour and biomarkers as tools to assess the acute toxicity of benzo[a]pyrene in the common prawn Palaemon serratus. Marine Environmental Research, 90: 39–46, DOI: 10.1016/j.marenvres.2013.05.010.CrossRef
  Torres, M. A., Barros, M. P., Campos, S. C., Pinto, E., Rajamani, S., Sayre, R. T., and Colepicolo, P., 2008. Biochemical biomarkers in algae and marine pollution: A review. Ecotoxicology and Environmental Safety, 71: 1–15, DOI: 10.1016/j.ecoenv.2008.05.009.CrossRef
  Van Oosterom, J., Codi King, S., Negri, A., Humphrey, C., and Mondon, J., 2010. Investigation of the mud crab (Scylla serrata) as a potential bio-monitoring species for tropical coastal marine environments of Australia. Marine Pollution Bulletin, 60(2): 283–290, DOI: 10.1016/j.marpolbul.2009.09. 007.CrossRef
  Van Schanke, A., Holtz, F., van der Meer, J. P., Boon, J. P., Ariese, F., Stroomberg, G., van den Berg, M., and Everaarts, J. M., 2001. Dose-and time-dependent formation of biliary benzo[a]pyrene metabolites in the marine flatfish dab (Limanda limanda). Environmental Toxicology and Chemistry, 20(8): 1641–1647.CrossRef
  Wang, Z. H., Nie, X. P., Yue, W. J., and Li, X., 2012. Physiological responses of three marine microalgae exposed to cypermethrin. Environmental Toxicology, 27(10): 563–572, DOI: 10.1002/tox.20678.CrossRef
  Wills, E. D., 1987. Evaluation of lipid perocidation in lipids and biological membranes. In: Biochemical Toxicology: A Practical Approach. Snell, K., and Mullock, B., eds., IRL Press, Washington, 127–152.
  Yang, X. L., Deng, S. Q., Philippis, R. D., Chen, L. Z., Hu, C. Z., and Zhang, W. H., 2012. Chemical composition of volatile oil from Artemisia ordosica and its allelopathiceffects on desert soil microalgae, Palmellococcus miniatus. Plant Physiology and Biochemistry, 51: 153–158, DOI: 10.1016/j.plaphy.2011. 10.019.CrossRef
  Zou, G. L., Gui, X. F., Zhong, X. L., and Zhu, R. P., 1986. Determination method of SOD-pyrogallol autoxidation improvement. Progress in Biochemistry and Biophysics, 4: 71–73 (in Chinese).
  
• 作者单位：Chen Shen (1)
  Jingjing Miao (1)
  Yun Li (1)
  Luqing Pan (1)
  
  1. Ministry of Education Key Laboratory of Mariculture, Ocean University of China, Qingdao, 266003, P. R. China
  
• 刊物主题：Oceanography; Meteorology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5021

文摘

The objective of this study was to examine the effect of benzo[a]pyrene (BaP) on the detoxification and antioxidant systems of two microalgae, Isochrysis zhanjiangensis and Platymonas subcordiformis. In our study, these two algae were exposed to BaP for 4 days at three different concentrations including 0.5 μg L−1 (low), 3 μg L−1 (mid) and 18 μg L−1 (high). The activity of detoxification enzymes, ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) increased in P. subcordiformis in all BaP-treated groups. In I. zhanjiangensis, the activity of these two enzymes increased at the beginning of exposure, and then decreased in the groups treated with mid- and high BaP. The activity of antioxidant enzyme superoxide dismutase (SOD) increased in I. zhanjiangensis in all BaP-treated groups, and then decreased in high BaP-treated group, while no significant change was observed in P. subcordiformis. The activity of antioxidant enzyme catalase (CAT) increased in I. zhanjiangensis and P. subcordiformis in all BaPtreated groups. The content of malondialdehyde (MDA) in Isochrysis zhanjiangensis increased first, and then decreased in high BaP-treated group, while no change occurred in P. subcordiformis. These results demonstrated that BaP significantly influenced the activity of detoxifying and antioxidant enzymes in microalgae. The metabolic related enzymes (EROD, GST and CAT) may serve as sensitive biomarkers of measuring the contamination level of BaP in marine water. Keywords benzo[a]pyrene marine microalga detoxifying enzyme antioxidant enzyme lipid peroxidation