Natural glufosinate resistance of soil microorganisms and GMO safety
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- 作者：Timea Tothova (1)
Anna Sobekova (2)
Katarina Holovska (2)
Jaroslav Legath (2)
Peter Pristas (1)
Peter Javorsky (1) - 关键词：Soil bacteria ; Glufosinate resistance ; Transgenic maize Bt 176 ; bar gene ; Horizontal gene transfer
- 刊名：Central European Journal of Biology
- 出版年：2010
- 出版时间：October 2010
- 年：2010
- 卷：5
- 期：5
- 页码：656-663
- 全文大小：1952KB
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Anna Sobekova (2)
Katarina Holovska (2)
Jaroslav Legath (2)
Peter Pristas (1)
Peter Javorsky (1)
1. Institute of Animal Physiology, Slovak Academy of Sciences, 040 01, Kosice, Slovakia
2. University of Veterinary Medicine, 041 81, Kosice, Slovakia - ISSN：1644-3632
文摘
Bacteria and fungi from pristine soil, never exposed to glufosinate herbicide, were isolated and analyzed for glufosinate tolerance. Seven of the 15 tested isolates were sensitive to 1 mM glufosinate (an active ingredient of many nonselective contact herbicides), 5 were resistant to 4 mM glufosinate and 3 even to 8 mM glufosinate in liquid medium. None of the isolated microorganisms carried the gene for glufosinate resistance bar (bialaphos resistance) in its genome and at least in some of glufosinate-resistant isolates the increased glutamine synthetase level was detected as a possible resistance mechanism. The transfer of the bar glufosinate resistance gene from transgenic maize Bt 176 into glufosinate-sensitive soil bacterium Bacillus pumilus S1 was not detected under the laboratory conditions by a classical plate count method and PCR. The ecological risk of potential bar gene transfer from genetically modified plants into soil microcosms under natural circumstances is discussed.