Comparison of three selectable marker genes for transformation of tall fescue (Festuca arundinacea Schreb.) plants by particle bombardment

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• 作者：Danfeng Long (1)
  Xueli Wu (2)
  Zhimin Yang (2)
  Ingo Lenk (3)
  Klaus Kristian Nielsen (3)
  Caixia Gao (4)
  
• 关键词：Tall fescue (Festuca arundinacea Schreb.) ; Particle bombardment ; Selectable markers ; Herbicide ; Antibiotic
• 刊名：In Vitro Cellular & Developmental Biology - Plant
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：47
• 期：6
• 页码：658-666
• 全文大小：541KB
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• 作者单位：Danfeng Long (1)
  Xueli Wu (2)
  Zhimin Yang (2)
  Ingo Lenk (3)
  Klaus Kristian Nielsen (3)
  Caixia Gao (4)
  
  1. School of Life Science, Lanzhou University, Lanzhou, China
  2. College of Horticulture, Nanjing Agricultural University, Nanjing, China
  3. Research Division, DLF-Trifolium Ltd., Hoejerupvej 31, Store Heddinge, Denmark
  4. The State Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
  

文摘

A variety of selection systems have been developed for transformation of forage crops. To compare the most frequently used systems, we tested three selectable marker genes for their selection efficiency under four selection procedures for the production of transgenic tall fescue. Embryogenic calluses initiated from mature embryos were bombarded with three constructs containing either the phosphinothricin acetyltransferase (bar) gene, the hygromycin phosphotransferase (hpt) gene or the neomycin phosphotransferase II (nptII) gene. Transformation efficiency was strongly influenced by the selectable marker gene, selection procedure and genotype. The highest transformation efficiency was observed using the bar gene in combination with bialaphos. Average transformation efficiencies with bialaphos, phosphinothricin (glufosinate), hygromycin and paromomycin selection across the two callus lines used in the experiments were 9.4%, 4.4%, 5.2% and 1.6%, respectively. Southern blot analysis revealed the independent nature of the tested transgenic plants and a complex transgene integration pattern with multiple insertions.