Production of bialaphos-resistant Nierembergia repens by electroporation

详细信息    查看全文

• 作者：Yoshiaki Shizukawa (1) (2)
  Masahiro Mii (1)
  
• 关键词：Electroporation ; Herbicide resistance ; Protoplast ; Nierembergia repens ; Solanaceae ; Transformation
• 刊名：Plant Biotechnology Reports
• 出版年：2008
• 出版时间：August 2008
• 年：2008
• 卷：2
• 期：3
• 页码：219-226
• 全文大小：485KB
• 参考文献：1. Anzai H, Ishii Y, Shichinohe M, Katsumata K, Nojiri C, Morikawa H, Tanaka M (1996) Transformation of phalaenopsis by particle bombardment. Plant Tissue Cult Lett 13:265-72
  2. Arita H, Hujii Y (1998) Ground cover plants efficiently employed in border region and a cultivated land. Nobunkyo, Tokyo (in Japanese)
  3. Asano Y, Ito Y, Fukami M, Sugiura K, Fujiie A (1998) Herbicide-resistant transgenic creeping bentgrass plants obtained by electroporation using an altered buffer. Plant Cell Rep 17:963-67 CrossRef
  4. Chen WP, Punja ZK (2002) Transgenic herbicide- and disease- tolerant carrot ( / Daucus carota L.) plants obtained through / Agrobacterium-mediated transformation. Plant Cell Rep 20:929-35 CrossRef
  5. Cocucci AA (1991) Pollination biology of / Nierembergia( / Solanaceae). Plant Syst Evol 174:947-53 CrossRef
  6. De Block M (1988) Genotype-independent leaf disc transformation of potato ( / Solanum tuberosum) using / Agrobacterium tumefaciens. Theor Appl Genet 76:767-74 CrossRef
  7. De Block M, Botterman J, Vandewiele M, Dockx J, Thoen C, Gossele V, Rao Movva N, Thompson C, Van Montagu M, Leemans J (1987) Engineering herbicide resistance in plants by expressing of detoxifying enzyme. EMBO J 6:2513-518
  8. De Block M, De Brouwer D, Tenning P (1989) Transformation of / Brassica napus and / Brassica oleracea using / Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol 91:694-01 CrossRef
  9. Dekeyser R, Clase B, Marichal M, Van Montagu M, Caplan A (1989) Evaluation of selectable markers for rice transformation Plant Physiol. 90:217-23
  10. Gao C, Jiang L, Folling M, Han L, Nielsen KK (2006) Generation of large numbers of transgenic Kentucky bluegrass ( / Poa pratensis L.) plants following biolistic gene transfer. Plant Cell Rep 25:19-5 CrossRef
  11. Ghosh M, Saha T, Nayak P, Sen SK (2002) Genetic transformation by particle bombardment of cultivated jute, / Corchorus capsularis L. Plant Cell Rep 20:936-42 CrossRef
  12. Godo T, Tsujii O, Ishikawa K, Mii M (1997) Fertile transient plants of / Nierembergia scoparia Sendtner obtained by a mikimopine type strain of / Agrobacterium rhizogenes. Sci Hortic 68:101-11 CrossRef
  13. Hartman CL, Lee L, Day PR, Tumer NE (1994) Herbicide resistant turfgrass ( / Agrostis palustris Huds.) by biolistic transformation. BioTechnology 12:919-23 CrossRef
  14. Hoshina R (1999) The present condition of border region management and vegetation management utilized the ground cover plant in Hiroshima Prefecture. Syokutyou 33:187-93 (in Japanese)
  15. Hoshino Y, Mii M (1998) Bialaphos stimulates shoot regeneration from hairy roots of snapdragon ( / Antirrhinum majus L.) transformed by / Agrobacterium rhizogenes. Plant Cell Rep 17:256-61 CrossRef
  16. Howe AR, Gasser CS, Brown SM, Padgette SR, Hart J, Parker GB, Fromm ME, Armstrong CL (2002) Glyphosate as a selective agent for the production of fertile transgenic maize ( / Zea mays L.) plants. Mol Breed 10:153-64 CrossRef
  17. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion maker in higher plants. EMBO J 6:3901-907
  18. Khan RS, Chin DP, Nakamura I, Mii M (2006) Production of marker-free transgenic / Nierembergia caerulea using MAT vector system. Plant Cell Rep 25:914-19 CrossRef
  19. Knapp JE, Kausch AP, Chandlee JM (2000) Transformation of three genera of orchid using the bar gene as a selectable marker. Plant Cell Rep 19:893-98 CrossRef
  20. Kohli A, Twyman RM, Abranches R, Wegel E, Stoger E, Christou P (2003) Transgene integration, organization and interaction in plants. Plant Mol Biol 52:247-58 CrossRef
  21. Murakami T, Anzai H, Imai S, Satho A, Nagaoka K, Thompson CJ (1986) The bialaphos biosynthetic genes of / Streptmyces hygroscopicus: molecular cloning and characterization of gene cluster. Mol Gen Genet 250:42-0 CrossRef
  22. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-97 CrossRef
  23. Osakabe K, Endo M, Kawai K, Nishizawa Y, Ono K, Abe K, Ishikawa Y, Nakamura H, Ichikawa H, Nishimura S (2005) The mutant form acetolactate synthase genomic DNA from rice is an efficient selectable marker for genetic transformation. Mol Breed 16:313-20 CrossRef
  24. Park MY, Yi NR, Lee HY, Kim ST, Kim M, Park JH, Kim JK, Cheong JJ, Choi YD (2002) Generation of chlorsulfuron-resistant transgenic garlic plants ( / Allium sativum L.) by particle bombardment. Mol Breed 9:171-81 CrossRef
  25. Roger SO, Bendich AJ (1985) Expression of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69-6 CrossRef
  26. Russell DR, Wallace KM, Bath JH, Martinell BJ, McCabe DE (1993) Stable transformation of Phaseolus vulgaris via electric-discharge mediated particle acceleration Plant Cell Rep. 12:165-69
  27. Saito K, Yamazaki M, Anzai H, Yoneyama K, Mourakoshi I (1992) Transgenic herbicide-resistant / Atropa belladonna using an Ri binary vector and inheritance of transgenic trait. Plant Cell Rep 11:219-24 CrossRef
  28. Shizukawa Y, Mii M (1997) A simple and efficient plant regeneration system for leaf protoplasts of / Nierembergia repens by inducing single shoots on the microcolonies. Plant Cell Rep 16:545-49
  29. Spencer TM, Gorden-Kamm WJ, Daines RJ, Start WG, Lemaux PG (1990) Bialaphos selection of stable transformants from maize cell culture. Theor Appl Genet 79:625-31 CrossRef
  30. Srinivasa Reddy MS, Dinkins RD, Collins GB (2003) Gene silencing in transgenic soybean plants transformed via particle bombardment. Plant Cell Rep 21:676-83
  31. Tachibana K, Kaneko K (1986) Development of new herbicide, bialaphos. J Pestic Sci 11:297-04
  32. Tada Y, Sakamoto M, Fujimura T (1990) Eficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions. Theor Appl Genet 80:475-80
  33. Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lanwereys M, Botterman J (1987) Characterization of the herbicide-resistance gene / bar from / Streptomyces hygroscopicus. EMBO J 6:2519-523
  34. Ueyama Y, Katsumoto Y, Fukui Y, Fukuchi-Mizutani M, Ohkawa H, Kusumi T, Iwashita T, Tanaka Y (2006) Molecular characterization of the flavonoid biosynthetic pathway. Plant Biotechnol 23:19-4
  35. Yamazaki M, Son L, Hayashi T, Morita N, Asamizu T, Mourakoshi I, Saito K (1996) Transgenic fertile / Scoparia dulcis L., a folk medicinal plant, conferred with a herbicide-resistant trait using an Ri binary vector. Plant Cell Rep 15:317-21 CrossRef
  
• 作者单位：Yoshiaki Shizukawa (1) (2)
  Masahiro Mii (1)
  
  1. Graduate School of Horticulture, Chiba University, Matsudo, Chiba, 271-8510, Japan
  2. Kyoto Prefectural Institute of Agricultural Biotechnology, 74 Ooji, Kitaine-yatsuma, Seika, Souraku, Kyoto, 619-0244, Japan
  

文摘

Transgenic plants with the herbicide-resistance gene (bar gene) were obtained via organogenesis from isolated mesophyll protoplasts of Nierembergia repens after applying electroporation. Transient β-glucuronidase (GUS) activity of electroporated protoplasts assayed 2?days after applying an electric pulse showed that optimum condition (transient GUS activity 319?pmol 4 MU/mg per min and plating efficiency 2.43%) for electroporation was 0.5 kV/cm in field strength and 100 μF in capacitance. The protoplasts electroporated with the bar gene at this condition initiated formation of microcolonies on medium after 2?weeks. After 4?weeks of culture, equal volume of fresh 1/2-strength Murashige and Skoog (MS) medium containing 0.2?mg/l bialaphos was added for selection of transformed colonies. After 6?weeks of culture, growing colonies were transferred onto regeneration medium containing 1.0?mg/l bialaphos, on which they formed adventitious shoots 1-?months after electroporation. The adventitious shoots rooted easily after transfer onto MS medium with bialaphos lacking plant-growth regulators. Transformation of these regenerants with the bar gene was confirmed by Southern analysis. Some of the transformants showed strong resistance to the application of bialaphos solution at 10.0?mg/l.