Application of Gaussia luciferase in bicistronic and non-conventional secretion reporter constructs

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• 作者：Christin Luft (1)
  Jamie Freeman (1)
  David Elliott (1)
  Nadia Al-Tamimi (1)
  Janos Kriston-Vizi (1)
  Jacob Heintze (1)
  Ida Lindenschmidt (1)
  Brian Seed (2)
  Robin Ketteler (1)
  
  1. Medical Research Council ; Laboratory for Moleclar and Cell Biology ; University College London ; Gower Street ; London ; WC1E 6BT ; UK
  2. Center for Computational and Integrative Biology ; Massachusetts General Hospital ; Boston ; MA02114 ; USA
  
• 关键词：Non ; conventional secretion ; Gaussia luciferase ; GGA1 ; Bicistronic expression ; Signal peptide
• 刊名：BMC Biochemistry
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：15
• 期：1
• 全文大小：1,841 KB
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• 刊物主题：Biochemistry, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2091

文摘

Background Secreted luciferases are highly useful bioluminescent reporters for cell-based assays and drug discovery. A variety of secreted luciferases from marine organisms have been described that harbor an N-terminal signal peptide for release along the classical secretory pathway. Here, we have characterized the secretion of Gaussia luciferase in more detail. Results We describe three basic mechanisms by which GLUC can be released from cells: first, classical secretion by virtue of the N-terminal signal peptide; second, internal signal peptide-mediated secretion and third, non-conventional secretion in the absence of an N-terminal signal peptide. Non-conventional release of dNGLUC is not stress-induced, does not require autophagy and can be enhanced by growth factor stimulation. Furthermore, we have identified the golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1) as a suppressor of release of dNGLUC. Conclusions Due to its secretion via multiple secretion pathways GLUC can find multiple applications as a research tool to study classical and non-conventional secretion. As GLUC can also be released from a reporter construct by internal signal peptide-mediated secretion it can be incorporated in a novel bicistronic secretion system.