Methionine deficiency reduces autophagy and accelerates death in intestinal epithelial cells infected with enterotoxigenic Escherichia coli

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• 作者：Yulong Tang ; Bie Tan ; Xia Xiong ; Fengna Li ; Wenkai Ren ; Xiangfeng Kong…
• 关键词：Apoptosis ; Autophagy ; Cytotoxicity ; Enterotoxigenic Escherichia coli ; IPEC ; 1 ; Methionine
• 刊名：Amino Acids
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：47
• 期：10
• 页码：2199-2204
• 全文大小：792 KB
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• 作者单位：Yulong Tang (1)
  Bie Tan (1)
  Xia Xiong (1)
  Fengna Li (1)
  Wenkai Ren (1)
  Xiangfeng Kong (1)
  Wei Qiu (2)
  Philip R. Hardwidge (3)
  Yulong Yin (1)
  
  1. Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Research Center of Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Changsha, Hunan, Peoples Republic of China
  2. Hunan New Wellful Co., Ltd, Changsha, 410001, Hunan, China
  3. Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS, 66506, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199

文摘

Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without l-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy. Keywords Apoptosis Autophagy Cytotoxicity Enterotoxigenic Escherichia coli IPEC-1 Methionine