Backbone 1H, 15N, 13C NMR assignment of the 518᾿27 fragment of the androgen receptor encompassing N-terminal and DNA binding domains

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• 作者：Sandra Meyer ; Ying-Hui Wang ; Pau Pérez-Escrivà…
• 关键词：Nuclear receptor ; Androgen receptor ; DNA binding domain
• 刊名：Biomolecular NMR Assignments
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：10
• 期：1
• 页码：175-178
• 全文大小：836 KB
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• 作者单位：Sandra Meyer (1)
  Ying-Hui Wang (1)
  Pau Pérez-Escrivà (1)
  Bruno Kieffer (1)
  
  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire, INSERM, U596, CNRS, UMR-7104, Université de Strasbourg, 1, rue Laurent Fries, 67404, Illkirch-Graffenstaden, France
  
• 刊物类别：Physics and Astronomy
• 刊物主题：None Assigned
• 出版者：Springer Netherlands
• ISSN：1874-270X

文摘

Androgen receptor (AR) belongs to the nuclear receptor superfamily that are ligand dependent transcription factors. This protein binds to steroid hormones such as dihydrotestosterone, to specific DNA sequences as well as to a number of co-regulatory factors. A number of these interactions involve the N-terminal domain (NTD), that is predicted to be intrinsically disordered. In order to provide functional information about possible cross-talk mechanisms between the AR NTD and its DNA binding domain (DBD), we have undertaken the NMR study of a fragment of human AR encompassing the last 37 residues of the NTD and the DBD (NTD–DBD_518–627). The backbone 1H, 15N, 13C NMR resonance assignments of this fragment indicate the presence of residual helical secondary structure within the AR NTD.