Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs

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• 作者：Zhonghua Shi ; Chun Zhao ; Ye Yang ; Hui Teng…
• 关键词：hnRNP E1 ; mRNA stabilization ; Pronuclear development ; Microinjection
• 刊名：Cellular and Molecular Life Sciences (CMLS)
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：72
• 期：18
• 页码：3575-3586
• 全文大小：1,333 KB
• 参考文献：1.Horner VL, Wolfner MF (2008) Transitioning from egg to embryo: triggers and mechanisms of egg activation. Dev Dyn 237:527-44. doi:10.-002/?dvdy.-1454 CrossRef PubMed
  2.Krauchunas AR, Wolfner MF (2013) Molecular changes during egg activation. Curr Top Dev Biol 102:267-92. doi:10.-016/?B978-0-12-416024-8.-0010-6 PubMed Central CrossRef PubMed
  3.Malcuit C, Kurokawa M, Fissore RA (2006) Calcium oscillations and mammalian egg activation. J Cell Physiol 206:565-73. doi:10.-002/?jcp.-0471 CrossRef PubMed
  4.Stitzel ML, Seydoux G (2007) Regulation of the oocyte-to-zygote transition. Science 316:407-08. doi:10.-126/?science.-138236 CrossRef PubMed
  5.Collas P (1998) Cytoplasmic control of nuclear assembly. Reprod Fertil Dev 10:581-92CrossRef PubMed
  6.Poccia D, Collas P (1997) Nuclear envelope dynamics during male pronuclear development. Dev Growth Differ 39:541-50CrossRef PubMed
  7.Perreault SD, Naish SJ, Zirkin BR (1987) The timing of hamster sperm nuclear decondensation and male pronucleus formation is related to sperm nuclear disulfide bond content. Biol Reprod 36:239-44CrossRef PubMed
  8.Clift D, Schuh M (2013) Restarting life: fertilization and the transition from meiosis to mitosis. Nat Rev Mol Cell Biol 14:549-62. doi:10.-038/?nrm3643 PubMed Central CrossRef PubMed
  9.Li L, Zheng P, Dean J (2010) Maternal control of early mouse development. Development 137:859-70. doi:10.-242/?dev.-39487 PubMed Central CrossRef PubMed
  10.Nakamura H, Wu C, Kuang J, Larabell C, Etkin LD (2000) XCS-1, a maternally expressed gene product involved in regulating mitosis in Xenopus. J Cell Sci 113(Pt 13):2497-505PubMed
  11.Ma M, Guo X, Wang F, Zhao C, Liu Z, Shi Z, Wang Y, Zhang P, Zhang K, Wang N, Lin M, Zhou Z, Liu J, Li Q, Wang L, Huo R, Sha J, Zhou Q (2008) Protein expression profile of the mouse metaphase-II oocyte. J Proteome Res 7:4821-830. doi:10.-021/?pr800392s CrossRef PubMed
  12.Wang S, Kou Z, Jing Z, Zhang Y, Guo X, Dong M, Wilmut I, Gao S (2010) Proteome of mouse oocytes at different developmental stages. Proc Natl Acad Sci USA 107:17639-7644. doi:10.-073/?pnas.-013185107 PubMed Central CrossRef PubMed
  13.Ko JL and Loh HH (2005) Poly C binding protein, a single-stranded DNA binding protein, regulates mouse mu-opioid receptor gene expression. J Neurochem 93:749-61. doi:10.-111/?j.-471-4159.-005.-3089.?x CrossRef PubMed
  14.Kim JH, Hahm B, Kim YK, Choi M, Jang SK (2000) Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. J Mol Biol 298:395-05. doi:10.-006/?jmbi.-000.-687 CrossRef PubMed
  15.Weiss IM, Liebhaber SA (1994) Erythroid cell-specific determinants of alpha-globin mRNA stability. Mol Cell Biol 14:8123-132PubMed Central PubMed
  16.Weiss IM, Liebhaber SA (1995) Erythroid cell-specific mRNA stability elements in the alpha 2-globin 3-nontranslated region. Mol Cell Biol 15:2457-465PubMed Central PubMed
  17.Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R and Regitz-Zagrosek V (2004) RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res 95:1058-066. doi:10.-161/-1.?RES.-000149166.-3833.-8 CrossRef PubMed
  18.Paulding WR, Czyzyk-Krzeska MF (1999) Regulation of tyrosine hydroxylase mRNA stability by protein-binding, pyrimidine-rich sequence in the 3-untranslated region. J Biol Chem 274:2532-538CrossRef PubMed
  19.Czyzyk-Krzeska MF, Bendixen AC (1999) Identification of the poly(C) binding protein in the complex associated with the 3-untranslated region of erythropoietin messenger RNA. Blood 93:2111-120PubMed
  20.Evans JR, Mitchell SA, Spriggs KA, Ostrowski J, Bomsztyk K, Ostarek D, Willis AE (2003) Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo. Oncogene 22:8012-020. doi:10.-038/?sj.?onc.-206645 CrossRef PubMed
  21.Pickering BM, Mitchell SA, Spriggs KA, Stoneley M, Willis AE (2004) Bag-1 internal ribosome entry segment activity is promoted by structural changes mediated by poly(rC) binding protein 1 and recruitment of polypyrimidine tract binding protein 1. Mol Cell Biol 24:5595-605. doi:10.-128/?MCB.-4.-2.-595-5605.-004 PubMed Central CrossRef PubMed
  22.Pickering BM, Mitchell SA, Evans JR, Willis AE (2003) Polypyrimidine tract binding protein and poly r(C) binding protein 1 interact with the BAG-1 IRES and stimulate its activity in vitro and in vivo. Nucleic Acids Res 31:639-46PubMed Central CrossRef PubMed
  23.Zhong N, Radu G, Ju W and Brown WT (2005) Novel progerin-interactive partner proteins hnRNP E1, EGF, Mel 18, and UBC9 interact with lamin A/C. Biochem Biophys Res Commun 338:855-61. doi:10.-016/?j.?bbrc.-005.-0.-20 CrossRef PubMed
  24.Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabasi AL, Vidal M and Zoghbi HY (
• 作者单位：Zhonghua Shi (1) (3)
  Chun Zhao (1) (3)
  Ye Yang (1) (3)
  Hui Teng (1)
  Ying Guo (1)
  Minyue Ma (1) (2)
  Xuejiang Guo (1)
  Zuomin Zhou (1)
  Ran Huo (1)
  Qi Zhou (2)
  
  1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, 210029, People’s Republic of China
  3. State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing, 210011, People’s Republic of China
  2. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Life Sciences
  Biochemistry
  
• 出版者：Birkh盲user Basel
• ISSN：1420-9071

文摘

In mammals, pronucleus formation, a landmark event for egg activation and fertilization, is critical for embryonic development. However, the mechanisms underlying pronucleus formation remain unclear. Increasing evidence has shown that the transition from a mature egg to a developing embryo and the early steps of development are driven by the control of maternal cytoplasmic factors. Herein, a two-dimensional-electrophoresis-based proteomic approach was used in metaphase II and parthenogenetically activated mouse eggs to search for maternal proteins involved in egg activation, one of which was poly(rC)-binding protein 1 (PCBP1). Phosphoprotein staining indicated that PCBP1 displayed dephosphorylation in parthenogenetically activated egg, which possibly boosts its ability to bind to mRNAs. We identified 75 mRNAs expressed in mouse eggs that contained the characteristic PCBP1-binding CU-rich sequence in the 3-UTR. Among them, we focused on H2a.x mRNA, as it was closely related to pronucleus formation in Xenopus oocytes. Further studies suggested that PCBP1 could bind to H2a.x mRNA and enhance its stability, thus promoting mouse pronucleus formation during parthenogenetic activation of murine eggs, while the inhibition of PCBP1 evidently retarded pronucleus formation. In summary, these data propose that PCBP1 may serve as a novel maternal factor that is required for determining the normal timing of pronucleus formation. Keywords hnRNP E1 mRNA stabilization Pronuclear development Microinjection