The use of additives as the stimulator on mycelial biomass and exopolysaccharide productions in submerged culture of Grifola umbellata

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• 作者：Hua-Bing Chen ; Hung-Chang Huang ; Chih-I Chen ; Yet-Pole I and Yung-Chuan Liu
• 关键词：Grifola umbellata ; Exopolysaccharide ; Polyethylene glycol ; Mycelial biomass ; Stimulatory effect
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2010
• 出版时间：March, 2010
• 年：2010
• 卷：33
• 期：3
• 页码：401-406
• 全文大小：326.5 KB

文摘

In this study, various additives including organic acids, alcohols, vegetable oils, surfactants and polymers were added in the cultural medium to investigate their stimulatory effects on Grifola umbellate mycelia growth and exopolysaccharide (EPS) production. It was found that the commonly used stimulatory additives, effective in other mushrooms’ cultures, exhibited negative results in Grifola umbellata submerged culture. In contrast, the polymer additive, polyethylene glycol (PEG), displayed an effective stimulatory effect on both biomass and EPS productions. With the addition of PEG8 (molecular weight: 8,000 Da), the mycelial biomass production at day 12 was increased from 4.69 to 6.30 g/L, accounting for a 34 % increase. Meanwhile, the EPS production was enhanced from 0.478 to 0.767 g/L, accounting for 60 % increase.