A marine sponge associated strain of Bacillus subtilis and other marine bacteria can produce anticholinesterase compounds
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- 作者：Sony Pandey (1)
Ayinampudi Sree (1)
Dipti Priya Sethi (1)
Chityal Ganesh Kumar (2)
Sudha Kakollu (2)
Lipsa Chowdhury (1)
Soumya Suchismita Dash (1) - 关键词：Acetylcholinesterase inhibitors ; Galanthamine ; Alzheimer’s disease ; Fasciospongia cavernosa ; Marine bacteria ; Screening ; Soft coral ; marine sediment
- 刊名：Microbial Cell Factories
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：13
- 期：1
- 全文大小：285 KB
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Ayinampudi Sree (1)
Dipti Priya Sethi (1)
Chityal Ganesh Kumar (2)
Sudha Kakollu (2)
Lipsa Chowdhury (1)
Soumya Suchismita Dash (1)
1. Environment and Sustainability Department, CSIR - Institute of Minerals and Materials Technology, Bhubaneswar, 751 013, India
2. Medicinal Chemistry and Pharmacology Division, CSIR- Indian Institute of Chemical Technology, Uppal Road, Hyderabad, 500 007, India - ISSN：1475-2859
文摘
Background Acetylcholinesterase (AChE) inhibitors or anticholinesterases reduce the activity of enzyme acetylcholinesterase that degrades the neurotransmitter acetylcholine in the brain. The inhibitors have a significant pharmacological role in neurodegenerative diseases like Alzheimer’s and Parkinson’s etc. Although plants have been a significant source of these compounds, there are very few sporadic reports of microorganisms producing such inhibitors. Anticholinesterase activity in bacterial associates of marine soft corals and sponges were not previously reported. Results We screened 887 marine bacteria for the presence of acetylcholinesterase inhibitors, in a microplate based assay, and found that 140 (15.8%) of them inhibit the electric eel enzyme, acetylcholinesterase. Majority of the active isolates were bacterial associates of soft corals followed by sediment isolates while most of the potent inhibitors belonged to the bacterial associates of marine sponges. Maximum inhibition (54%) was exhibited by a bacterial strain M18SP4P (ii), isolated from the marine sponge Fasciospongia cavernosa. Based on phenotypic characterization and 16S rDNA sequencing, the strain was identified as Bacillus subtilis - revealing yet another activity in a strain of the model organism that is considered to be a cell factory. TLC bioautography of the methanol extract of this culture, showed the presence of two major components having this activity, when compared to Galanthamine, the positive control. Conclusion From the results of our study, we conclude that acetylcholinesterase inhibitors are quite prevalent in marine bacteria, particularly the bacterial associates of marine invertebrates. Several potential AChE inhibitors in marine bacteria are waiting to be discovered to provide easily manipulable natural sources for the mass production of these therapeutic compounds.