Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli

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• 作者：Paul Quehl ; Joel Hollender ; Jan Schüürmann ; Tatjana Brossette…
• 关键词：Surface display ; Autotransporter ; Autodisplay ; Cytochrome P450 1A2 ; Cytochrome P450 reductase ; Whole cell biocatalysis
• 刊名：Microbial Cell Factories
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：15
• 期：1
• 全文大小：1,725 KB
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• 作者单位：Paul Quehl (1)
  Joel Hollender (1) (2)
  Jan Schüürmann (1)
  Tatjana Brossette (2)
  Ruth Maas (2)
  Joachim Jose (1)
  
  1. Institut für Pharmazeutische und Medizinische Chemie, PharmaCampus, Westfälische Wilhelms-Universität Münster, Corrensstraße 48, 48149, Münster, Germany
  2. Autodisplay Biotech GmbH, Merowingerplatz 1a, 40225, Düsseldorf, Germany
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Applied Microbiology
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1475-2859

文摘

Background Human cytochrome P450 (CYP) enzymes mediate the first step in the breakdown of most drugs and are strongly involved in drug–drug interactions, drug clearance and activation of prodrugs. Their biocatalytic behavior is a key parameter during drug development which requires preparative synthesis of CYP related drug metabolites. However, recombinant expression of CYP enzymes is a challenging bottleneck for drug metabolite biosynthesis. Therefore, we developed a novel approach by displaying human cytochrome P450 1A2 (CYP1A2) and cytochrome P450 reductase (CPR) on the surface of Escherichia coli.