Selective enrichment and production of highly urease active bacteria by non-sterile (open) chemostat culture
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- 作者：Liang Cheng ; Ralf Cord-Ruwisch
- 关键词：Chemostat ; Urease ; Non ; sterile ; Activated sludge ; Biocementation ; Soil stabilization
- 刊名：Journal of Industrial Microbiology and Biotechnology
- 出版年：2013
- 出版时间：October 2013
- 年：2013
- 卷：40
- 期：10
- 页码：1095-1104
- 全文大小：508KB
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Ralf Cord-Ruwisch (1)
1. School of Biological Sciences and Biotechnology, Murdoch University, 90 South Street, Perth, WA, 6150, Australia - ISSN：1476-5535
文摘
In general, bioprocesses can be subdivided into naturally occurring processes, not requiring sterility (e.g., beer brewing, wine making, lactic acid fermentation, or biogas digestion) and other processes (e.g., the production of enzymes and antibiotics) that typically require a high level of sterility to avoid contaminant microbes overgrowing the production strain. The current paper describes the sustainable, non-sterile production of an industrial enzyme using activated sludge as inoculum. By using selective conditions (high pH, high ammonia concentration, and presence of urea) for the target bacterium, highly active ureolytic bacteria, physiologically resembling Sporosarcina pasteurii were reproducibly enriched and then continuously produced via chemostat operation of the bioreactor. When using a pH of 10 and about 0.2?M urea in a yeast extract-based medium, ureolytic bacteria developed under aerobic chemostat operation at hydraulic retention times of about 10?h with urease levels of about 60?μmol?min??ml? culture. For cost minimization at an industrial scale the costly protein-rich yeast extract medium could be replaced by commercial milk powder or by lysed activated sludge. Glutamate, molasses, or glucose-based media did not result in the enrichment of ureolytic bacteria by the chemostat. The concentration of intracellular urease was sufficiently high such that the produced raw effluent from the reactor could be used directly for biocementation in the field.