Molecular dynamics simulation of a psychrophilic adenylate kinase

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• 作者：Euiyoung Bae (1) (2) (4)
  Sojin Moon (1)
  George N. Phillips Jr. (2) (3)
  
  1. Department of Agricultural Biotechnology ; Seoul National University ; Seoul ; 151-921 ; Republic of Korea
  2. Department of Biochemistry ; University of Wisconsin-Madison ; Madison ; WI ; 53706 ; USA
  4. Center for Food and Bioconvergence ; and Research Institute of Agriculture and Life Sciences ; Seoul National University ; Seoul ; 151-921 ; Republic of Korea
  3. Department of Biochemistry and Cell Biology ; Rice University ; Houston ; TX ; 77005 ; USA
  
• 关键词：Adenylate kinase ; Enzyme ; Molecular dynamics simulation ; Protein ; Psychrophile
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：58
• 期：2
• 页码：209-212
• 全文大小：280 KB
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• 刊物主题：Applied Microbiology; Bioorganic Chemistry; Biological Techniques;
• 出版者：Springer Netherlands
• ISSN：2234-344X

文摘

Proteins from psychrophilic organisms can function at low temperatures and have shown potential for industrial applications. In the present study, thermal denaturation of a psychrophilic adenylate kinase (AKpsychro) from Bacillus globisporus was measured using circular dichroism spectroscopy and its molecular dynamics (MD) simulations were performed. The results from the unfolding experiment and MD simulations of AKpsychro were compared with those of its mesophilic and thermophilic homologues to study the relationship between dynamic motion and the cold adaptation of proteins.