Evaluations of chitosan/poly(D,L-lactic-co-glycolic acid) composite fibrous scaffold for tissue engineering applications

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• 作者：Soo Jung Kim (11110) (21110)
  Dae Hyeok Yang (11110)
  Heung Jae Chun (11110) (21110)
  Gue Tae Chae (31110)
  Ju Woong Jang (41110)
  Young Bock Shim (41110)
  
• 关键词：chitosan ; poly(lactic ; co ; glycolic acid) ; co ; electrospinning ; non ; woven fibrous scaffold ; cell proliferation ; foreign body reaction
• 刊名：Macromolecular Research
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：21
• 期：8
• 页码：931-939
• 全文大小：3356KB
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• 作者单位：Soo Jung Kim (11110) (21110)
  Dae Hyeok Yang (11110)
  Heung Jae Chun (11110) (21110)
  Gue Tae Chae (31110)
  Ju Woong Jang (41110)
  Young Bock Shim (41110)
  
  11110. Institute of Cell & Tissue Engineering, The Catholic University of Korea, Seoul, 137-701, Korea
  21110. Department of Biomedical Sciences, The Catholic University of Korea, Seoul, 137-701, Korea
  31110. Institute of Hansen鈥檚 Disease, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea
  41110. Research Institute of Biomedical Engineering, Korea Bone Bank Co. Ltd., Seoul, 153-782, Korea
  
• ISSN：2092-7673

文摘

The objective of the present study was to evaluate non-woven chitosan/poly(lactic-co-glycolic acid) fibrous scaffold (chitosan/PLGA FS) prepared by a co-electrospinning process for tissue engineering applications, as compared to chitosan and PLGA FSs. The morphological, structural, and mechanical properties of the FSs were assessed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy-attenuated total reflection mode (FTIR-ATR), and a universal testing machine (UTM). The biocompatibility of the FSs was also evaluated in vitro in cultures of mouse fibroblasts and in vivo by subcutaneous implantation studies in rats. SEM image of the chitosan/PLGA FS showed morphological similarities to the natural ECM, characterized by high surface-to-volume ratio, high porosity, and variable pore size distributions. The FTIR-ATR spectrum of chitosan/PLGA FS revealed incorporation of the characteristic bands of chitosan and PLGA, indicating the co-existence of two fibrous structures. The poor mechanical properties of chitosan FS was improved by co-electrospinning with PLGA. In vitro L929 cell proliferation assay revealed that the cytocompatibility of chitosan/PLGA FS was increased compared to that of PLGA. In addition, chitosan/PLGA FS showed the improved ability to resolve foreign body reactions compared to PLGA FS due to the presence of chitosan.