Investigation of the links between heterocyst and biohydrogen production by diazotrophic cyanobacterium A. variabilis ATCC 29413

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• 作者：Siti Fatihah Salleh ; Azlina Kamaruddin ; Mohamad Hekarl Uzir…
• 关键词：Azatryptophan ; Heterocyst ; Cyanobacteria ; Hydrogen ; Nitrogenase
• 刊名：Archives of Microbiology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：198
• 期：2
• 页码：101-113
• 全文大小：2,007 KB
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• 作者单位：Siti Fatihah Salleh (1)
  Azlina Kamaruddin (1)
  Mohamad Hekarl Uzir (1)
  Khairiah Abd Karim (1)
  Abdul Rahman Mohamed (1)
  
  1. Low Carbon Economy (LCE) Research Group, School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Seri Ampangan, 14300, Nibong Tebal, Seberang Prai Selatan, Pulau Pinang, Malaysia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

This work investigates the effect of heterocyst toward biohydrogen production by A. variabilis. The heterocyst frequency was artificially promoted by adding an amino acid analog, in this case DL-7-azatryptophan into the growth medium. The frequency of heterocyst differentiation was found to be proportional to the concentration of azatryptophan (0–25 µM) in the medium. Conversely, the growth and nitrogenase activity were gradually suppressed. In addition, there was also a distinct shortening of the cells filaments and detachment of heterocyst from the vegetative cells. Analysis on the hydrogen production performance revealed that both the frequency and distribution of heterocyst in the filaments affected the rate of hydrogen production. The highest hydrogen production rate and yield (41 µmol H₂ mg chl a−1 h−1 and 97 mL H₂ mg chl a−1, respectively) were achieved by cells previously grown in 15 µM of azatryptophan with 14.5 % of heterocyst frequency. The existence of more isolated heterocyst has been shown to cause a relative loss in nitrogenase activity thus lowering the hydrogen production rate. Keywords Azatryptophan Heterocyst Cyanobacteria Hydrogen Nitrogenase