Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules

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• 作者：Chengxin Luan ; Zixue Yang ; Baoan Chen
• 刊名：Journal of Fluorescence
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：26
• 期：3
• 页码：1131-1139
• 全文大小：383 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biophysics and Biomedical Physics
  Biotechnology
  Biochemistry
  Analytical Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4994
• 卷排序：26

文摘

For analysis of biomacromolecules, a sensitive, specified and reliable method is indispensable. Fluorescent dyes or fluorophores have been widely used as mediums to obtain readout signals in various assays or bioimaging because of their versatilities such as biocompatibility. Those fluorescent dyes based techniques manipulate many molecular interactions for analysis of biomacromolecules including antibody-protein interaction, base complementation, glycan-lectin interaction, etc. The strategies to manipulate those molecular interactions are various and always updating due to the development of biotechnological tools and instruments. In this minireview, we summarize the state of the art of signal improvement techniques for fluorescence detection of biomacromolecules especially proteins and nucleic acids. We focus on the principle and mechanism of those techniques for fluorescence detection of biomacromolecules. We also discuss the future trend of the techniques for fluorescence detection of biomacromolecules.KeywordsBiomacromoleculeSignal-to-noise ratioSignal improvementFluorescence