Bioproduction of phenylethanoid glycosides by plant cell culture of Scrophularia striata Boiss.: from shake-flasks to bioreactor

详细信息    查看全文

• 作者：Sedigheh Ahmadi-Sakha ; Mohsen Sharifi…
• 关键词：Acteoside ; Bioreactor ; Echinacoside ; Plant cell culture ; S. striata Boiss.
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：124
• 期：2
• 页码：275-281
• 全文大小：613 KB
• 参考文献：Ardeshiry LA, Rezaei Tavirani M, Mortazavi SA, Barzegar M, Moghadamnia SH, Rezaee MB (2010) Study of anti cancer property of Scrophularia striata extract on the human astrocytoma cell line (1321). IJPR 9:403–410
  Azadmehr A, Afshari A, Baradaran B, Hajiaghaee R, Rezazadeh S, Monsef-Esfahani H (2009) Suppression of nitric oxide production in activated murine peritoneal macrophages in vitro and ex vivo by Scrophularia striata ethanolic extract. J Ethnopharmacol 6:166–169CrossRef
  Babri S, Doosti MH, Fatehi L, Salari AA (2012) The effects of Scrophularia striata extract on anxiety and depression behaviors in adult male mice. Pharma Sci 18(2):133–140
  Bahrami AM, Valadi A (2010) Effect of Scrophularia striata ethanolic leaves extracts on staphylococcus areus. Int J Pharmacol 6(4):431–434CrossRef
  Chattopadhyay S, Srivastava AK, Bhojwani SS, Bisaria VS (2002) Production of podophyllotoxin by plant cell cultures of Podophyllum hexandrum in bioreactor. J Biol Sci Bioeng 93(2):215–220CrossRef
  Chen WH, Xu CM, Zeng JL, Zhao B, Wang XD, Wang YC (2007) Improvement of echinacoside and acteoside production by two-stage elicitation in cell suspension culture of Cistanche deserticola. World J Microbiol Biotechnol 23:1451–1458CrossRef
  Estrada-Zuniga ME, Cruz-Sosa F, Rodríguez-Monroy M, Verde-Calvo JR, Vernon-Carter EJ (2009) Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth. Plant Cell Tissue Organ Cult 97:39–47CrossRef
  Georgiev M, Ludwig-Müller J, Weber J, Stancheva N, Bley T (2011) Bioactive metabolite production and stress-related hormones in Devil’s claw cell suspension cultures grown in bioreactors. Appl Microbiol Biotechnol 89:1683–1691CrossRef PubMed
  Jia C, Shi H, Jin W, Zhang K, Jiang Y, Zhao M, Tu P (2009) Metabolism of echinacoside, a good antioxidant, in rats: isolation and identification of itd Biliary metabolites. Drug Metab Dispos 37:431–438CrossRef PubMed
  Khanpour-Ardestani N, Sharifi M, Behmanesh M (2014) Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production. Cytotechnology. doi:10.​1007/​s10616-014-9705-4 PubMed
  Li L, Chung-ming L, Zhao-jie C, Jing W, Dong-fang S, Zhi-qiang L (2009) Isolation and purification of plantamajoside and acteoside from plant extract of Plantago asiatica L. by high performance centrifugal partition chromatography. Chem Res Chin Univ 25(6):817–821
  Mathew Annie J, Jayachandran K (2009) Production of scopadulcic acid B from Scoparia dulcis Linn. using a Luffa sponge bioreactor. Plant Cell Tissue Organ Cult 98:197–203CrossRef
  Monsef-Esfahani HR, Hajiaghaee R, Shahverdi AR, Khorramizadeh MR, Amini M (2010) Flavonoids, cinnamic acid and phenylpropanoid from aerial parts of Scrophularia striata. Pharm Biol 48(3):333–336CrossRef PubMed
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497CrossRef
  Ouyang J, Wang XD, Zhao B, Wang YC (2005a) Improved production of phenylethanoid glycosides by Cistanche deserticola cells cultured in an internal loop airlift bioreactor with sifter riser. Enzyme Microb Technol 36:982–988CrossRef
  Ouyang J, Wang XD, Zhao B, Wang YC (2005b) Enhanced production of phenylethanoid glycosides by precursor feeding to cell culture of Cistanche deserticola rocess. Biochemistry 40:3480–3484
  Rodriguez-Monroy M, Galindo E (1999) Broth rheology, growth and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shake flasks and in stirred tank. Enzyme Microb Technol 24:687–693CrossRef
  Safavi F, Meighani H, Ebrahimi P, Hafez Ghoran S (2012) Antioxidant and antibacterial activity of Scrophularia striata. Res Pharm Sci 7(5):S582
  Saimaru H, Orihara Y (2010) Biosynthesis of acteoside in cultured cells of Olea europaea. J Nat Med 64:139–145CrossRef PubMed
  Sharma V, Goyal S, Ramawat KG (2009) Scale up production of grown in shake flasks and bioreactor. Eng Life Sci 9:267–271CrossRef
  Shoohani B, Hemati AA, Taheri Moghadam M (2010) Effects of Scrophularia striata extract on wound healing in rabbit. J Ilam Univ Med Sci 17(4):9–16
  Suresh B, Rajasekaran T, Ramachandra Rao S, Raghavarao KSMS, Ravishankar GA (2001) Studies on osmolarity, conductivity and mass transfer for selection of a bioreactor for Tagets patula L. hairy roots. Process Biochem 36:987–993CrossRef
  Terrier B, Courtois D, Henault N, Cuvier A, Bastin M, Aknin A, Dubreuil J, Petiard V (2007) Two new disposable bioreactors for plant cell culture: the wave and undertow bioreactor and the slug bubble bioreactor. Biotechnol Bioeng 96:914–923CrossRef PubMed
  Vertuani S, Beghelli E, Scalambra E, Malisardi G, Copetti S, Toso RD, Baldisserotto A, Manfredini S (2011) Activity and stability studies of verbascoside, a novel antioxidant, in dermo-cosmetic and pharmaceutical topical formulations. Molecules 16:7068–7080CrossRef PubMed
  Wang GR, Qi NM, Wang ZM (2010) Application of a stir-tank bioreactor for perfusion culture and continuous harvest of Glycyrrhiza inflate suspension cells. Afr J Biotechnol 9:347–351
  
• 作者单位：Sedigheh Ahmadi-Sakha (1)
  Mohsen Sharifi (1)
  Vahid Niknam (2)
  
  1. Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  2. School of Biology, Center for Excellence in Phylogeny of Living Organisms in Iran, College of Science, University of Tehran, Tehran, Iran
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Phenylethanoid glycosides (PeG) are a class of polyphenols found in some plants that have pharmaceutical effects as anti-inflammatories and anti-oxidants. The presence of PeG (acteoside) in the aerial parts of Scrophularia striata Boiss. has been demonstrated. Considerable progress has been made using plant cell cultures to stimulate formation and accumulation of secondary metabolites. The present study optimized phenylethanoid production from shake flasks to bioreactor using a cell culture of S. striata. The optimal conditions for production of cell biomass by scale-up to a bioreactor were determined to be a pH of 4.8, air flow rate of 0.5–1.5 l min−1, and mixing speed of 110–170 rpm at 25 ± 1 °C in darkness. Growth parameters and PeG production were measured and compared with the results from the shake flasks. The results showed that cell biomass was high in the bioreactor (15.64 g l−1 DW) and in the shake flasks (14.16 g l−1 DW). The acteoside content in the bioreactor was 1404.20 μg g−1 DW, which is threefold higher than in the shake flasks (459.71 μg g−1 DW). The echinacoside concentration in the bioreactor was 1449.39 μg g−1, 1.36-fold lower than in the shake flasks (1973.03 μg g−1 DW). This study established an efficient way for production of acteoside, the major PeG, in a bioreactor.