Assessment of field-grown cellulase-expressing corn

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• 作者：Martina Garda (1)
  Shivakumar P. Devaiah (1) (5)
  Deborah Vicuna Requesens (1)
  Yeun-Kyung Chang (1) (6)
  Audrei Dabul (1) (7)
  Christy Hanson (2)
  Kendall R. Hood (3)
  Elizabeth E. Hood (1) (4)
  
  1. Arkansas Biosciences Institute ; Arkansas State University ; PO Box 639 ; State University ; AR ; 72467 ; USA
  5. Department of Biology ; East Tennessee State University ; Johnson City ; TN ; 37604 ; USA
  6. Institute for Advanced Learning and Research ; VA Tech ; Danville ; VA ; 24540 ; USA
  7. Dow AgroSciences ; Rod. Anhanguera Km 296 ; Cravinhos ; S茫o Paulo ; 14140-000 ; Brazil
  2. EPL Bio Analytical Services (EPL BAS) ; 9095聽W. Harristown Blvd. ; Niantic ; IL ; 62551 ; USA
  3. Infinite Enzymes ; LLC ; PO Box 2654 ; State University ; AR ; 72467 ; USA
  4. College of Agriculture and Technology ; Arkansas State University ; PO Box 1080 ; State University ; AR ; 72467 ; USA
  
• 关键词：Zea mays L. ; Recombinant protein ; Cellulases ; Industrial enzymes ; Substantial equivalence ; Field trial
• 刊名：Transgenic Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：24
• 期：2
• 页码：185-198
• 全文大小：3,011 KB
• 参考文献：1. Baker, J, Ehrman, C, Adney, W, Thomas, S, Himmel, M (1998) Hydrolysis of cellulose using ternary mixtures of purified celluloses. Appl Biochem Biotechnol 70鈥?2: pp. 395-403 CrossRef
  2. Bradford, KJ, Deynze, A, Gutterson, N, Parrott, W, Strauss, SH (2005) Regulating transgenic crops sensibly: lessons from plant breeding, biotechnology and genomics. Nat Biotechnol 23: pp. 439-444 CrossRef
  3. Castle, LA, Wu, G, McElroy, D (2006) Agricultural input traits: past, present and future. Curr Opin Biotechnol 17: pp. 105-112 CrossRef
  4. Church, GM, Gilbert, W (1984) Genomic sequencing. Proc Natl Acad Sci 81: pp. 1991-1995 CrossRef
  5. Fischer, R, Stoger, E, Schillberg, S, Christou, P, Twyman, RM (2004) Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7: pp. 152-158 CrossRef
  6. Fraley RT (2009) Molecular genetic approaches to maize improvement鈥揳n introduction. In: Kriz AL, Larkins BA (eds) Molecular genetic approaches to maize improvement, vol 63. Springer, Berlin Heidelberg, pp 3鈥?
  7. Hayden, C, Fake, G, Carroll, J, Hood, E, Howard, J (2012) Synergistic activity of plant extracts with microbial cellulases for the release of free sugars. Bioenergy Res 5: pp. 398-406 CrossRef
  8. Herman, RA, Price, WD (2013) Unintended compositional changes in genetically modified (gm) Crops: 20聽years of research. J Agric Food Chem 61: pp. 11695-11701 CrossRef
  9. Hood EE, Howard JA (2009) Over-expression of novel proteins in maize. In: Kriz AL, Larkins BA (eds) Molecular genetic approaches to maize improvement. Springer, Berlin Heidelberg, pp 91鈥?05
  10. Hood, E, Witcher, D, Maddock, S, Meyer, T, Baszczynski, C (1997) Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extracting, and purification. Mol Breed 3: pp. 291-306 CrossRef
  11. Hood, EE, Bailey, MR, Beifuss, K, Magallanes-Lundback, M, Horn, ME, Callaway, E, Drees, C, Delaney, DE, Clough, R, Howard, JA (2003) Criteria for high-level expression of a fungal laccase gene in transgenic maize. Plant Biotechnol J 1: pp. 129-140 CrossRef
  12. Hood, EE, Love, R, Lane, J, Bray, J, Clough, R, Pappu, K, Drees, C, Hood, KR, Yoon, S, Ahmad, A, Howard, JA (2007) Subcellular targeting is a key condition for high-level accumulation of cellulase protein in transgenic maize seed. Plant Biotechnol J 5: pp. 709-719 CrossRef
  13. Hood, EE, Devaiah, SP, Fake, G, Egelkrout, E, Teoh, K, Requesens, DV, Hayden, C, Hood, KR, Pappu, KM, Carroll, J, Howard, JA (2012) Manipulating corn germplasm to increase recombinant protein accumulation. Plant Biotechnol J 10: pp. 20-30 CrossRef
  14. Howard, JA, Donnelly, KC (2004) A quantitative safety assessment model for transgenic protein products produced in agricultural crops. J Agric Environ Ethics 17: pp. 545-558 CrossRef
  15. Howard, JA, Hood, E (2005) Bioindustrial and biopharmaceutical products produced in plants. Adv Agron 85: pp. 91-124 CrossRef
  16. Miller, JK, Bradford, K (2010) The regulatory bottleneck for biotech specialty crops. Nat Biotechnol 28: pp. 1012-1014 CrossRef
  17. Pollack A (2011) US approves corn modified for ethanol. The New York Times (February 11, B1)
  18. Ratner, M (2010) Pfizer stakes a claim in plant cell-made biopharmaceuticals. Nat Biotechnol 28: pp. 107-108 CrossRef
  19. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503鈥?17
  20. Sparrow, P, Broer, I, Hood, EE, Eversole, K, Hartung, F, Schiemann, J (2013) Risk assessment and regulation of molecular farming-a comparison between Europe and US. Curr Pharm Des 19: pp. 5513-5530 CrossRef
  21. Woodard, S, Mayor, J, Bailey, M, Barker, D, Love, R, Lane, J, Delaney, D, McComas-Wagner, J, Mallubhotla, H, Hood, E (2003) Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants. Biotechnol Appl Biochem 38: pp. 123-130 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Molecular Medicine
  Plant Genetics and Genomics
  Animal Genetics and Genomics
  Plant Sciences
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-9368

文摘

Transgenic plants in the US and abroad generated using genetic engineering technology are regulated with respect to release into the environment and inclusion into diets of humans and animals. For crops incorporating pharmaceuticals or industrial enzymes regulations are even more stringent. Notifications are not allowed for movement and release, therefore a permit is required. However, growing under permit is cumbersome and more expensive than open, non- regulated growth. Thus, when the genetically engineered pharmaceutical or industrial crop is ready for scale-up, achieving non-regulated status is critical. Regulatory compliance in the US comprises petitioning the appropriate agencies for permission for environmental release and feeding trials. For release without yearly permits, a petition for allowing non-regulated status can be filed with the United States Department of Agriculture with consultations that include the Food and Drug Administration and possibly the Environmental Protection Agency, the latter if the plant includes an incorporated pesticide. The data package should ensure that the plants are substantially equivalent in every parameter except for the engineered trait. We undertook a preliminary study on transgenic maize field-grown hybrids that express one of two cellulase genes, an exo-cellulase or an endo-cellulase. We performed field observations of whole plants and numerous in vitro analyses of grain. Although some minor differences were observed when comparing genetically engineered hybrid plants to control wild type hybrids, no significant differences were seen.