Mediated bioelectrochemical system for biosensing the cell viability of Staphylococcus aureus
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- 作者：Rabeay Y. A. Hassan ; Ulla Wollenberger
- 关键词：Microbial electrochemistry ; Pathogenic detection ; Probing living Staphylococcus aureus ; CNTs ; based screen printed electrodes
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：408
- 期：2
- 页码：579-587
- 全文大小：1,272 KB
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Ulla Wollenberger (1)
1. Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknechtstrasse 24-25, 14476, Potsdam-Golm, Germany
2. Microanalysis Lab, Applied Organic Chemistry Department, National Research Centre (NRC), El Bohouth St., Dokki, 12622, Giza, Egypt - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Food Science
Inorganic Chemistry
Physical Chemistry
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Berlin / Heidelberg
- ISSN：1618-2650
文摘
Staphylococcus aureus is one of the most dangerous human pathogens and is the cause of numerous illnesses ranging from moderate skin infections to life-threatening diseases. Despite advances made in identifying microorganisms, rapid detection methods for the viability of bacteria are still missing. Here, we report a rapid electrochemical assay for cell viability combining the use of double redox mediators and multiwall carbon nanotubes-screen printed electrodes (MWCNTs-SPE), ferricyanide (FCN) and 2,6-dichlorophenolindophenol (DCIP), which served as electron shuttle to enable the bacterial-electrode communications. The current originating from the metabolically active cells was recorded for probing the activity of the intracellular redox centers. Blocking of the respiratory chain pathways with electron transfer inhibitors demonstrated the involvement of the electron transport chain in the reaction. A good correlation between the number of the metabolically active cells and the current was obtained. The proposed assay has been exploited for monitoring cell proliferation of S. aureus during the growth. The sensitivity of the detection method reached 0.1 OD600. Therefore, the technique described is promising for estimating the cell number, measuring the cell viability, and probing intracellular redox center(s). Keywords Microbial electrochemistry Pathogenic detection Probing living Staphylococcus aureus CNTs-based screen printed electrodes