A Novel Optical Biosensing System Using Mach–Zehnder-Type Optical Waveguide for Influenza Virus Detection

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• 作者：Hiroaki Sakamoto ; Yuma Minpou ; Takayuki Sawai…
• 关键词：Molecular immobilization ; Optical biosensing ; Influenza virus detection
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：178
• 期：4
• 页码：687-694
• 全文大小：679 KB
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• 作者单位：Hiroaki Sakamoto (1) (5)
  Yuma Minpou (2)
  Takayuki Sawai (3) (5)
  Yasufumi Enami (4)
  Shin-ichiro Suye (3) (5)
  
  1. Tenure-Track Program for Innovation Research, University of Fukui, Fukui, Japan
  5. Research and Education Program for Life Science, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan
  2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui, Japan
  3. Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan
  4. Optoelectonic Engineering, School of Systems Engineering, Kochi University of Technology, Kochi, Japan
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Biochemistry
  
• 出版者：Humana Press Inc.
• ISSN：1559-0291

文摘

In order to minimize the damage from viral epidemics, early detection of the causative agent of a viral epidemic and prevention of its immediate spread are urgent social demands. Therefore, in this study, we evaluated the utility of a Mach–Zehnder-type optical waveguide as a sensing device for influenza virus detection. However, it is impossible to detect a 100-nm-size virus using a sol-gel optical biosensor because sol-gel glass has a pore size of only a few nanometers, which makes it impossible for the virus to diffuse into the silica thin film. In order to construct the influenza-specific Mach–Zehnder optical biosensor for influenza detection, a stable antibody immobilization method with resulting high density on the sol-gel surface is strongly required. In this study, the sol-gel glass surface was modified with amino and carboxyl groups, and an anti-H1N1/HA1 antibody was covalently immobilized using a cross-linking agent. We successfully prepared a carboxyl-modified sol-gel surface, using NHS/EDC as the cross-linker, for antibody immobilization, and confirmed the detection of influenza virus using the antibody-immobilized sol-gel glass. After treatment with a 100 μg/mL influenza virus solution for 15 min, a peak wavelength shift (~24 nm) was observed in the output light spectrum.