A new phosphothreonine lyase electrochemical immunosensor for detecting Salmonella based on horseradish peroxidase/GNPs-thionine/chitosan
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- 作者：Dingqiang Lu ; Guangchang Pang ; Junbo Xie
- 关键词：Phosphothreonine lyase ; Salmonella ; Electrochemical immunosensor ; Salmonella plasmid virulence C
- 刊名：Biomedical Microdevices
- 出版年：2017
- 出版时间：March 2017
- 年：2017
- 卷：19
- 期：1
- 全文大小：
- 刊物类别：Engineering
- 刊物主题：Biomedical Engineering; Biological and Medical Physics, Biophysics; Nanotechnology; Engineering Fluid Dynamics;
- 出版者：Springer US
- ISSN：1572-8781
- 卷排序：19
文摘
In the current study, a novel double-layer gold nanoparticles- electrochemical immunosensor electrode (DGN-EIE) immobilized with Salmonella plasmid virulence C (SpvC) antibody was developed. To increase the fixed quantity of antibodies and electrochemical signal, an electrochemical biosensing signal amplification system was utilized with gold nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). In addition, the SpvC monoclonal antibodies (derived from Balb/c mice) were prepared and screened with a high affinity to SpvC. To evaluate the quality of DGN-EIE, the amperometric I-t curve method was applied to determine Salmonella in PBS. The results showed that the response current had a good linear correlation with the bacterial quantity ranged from 1.0 × 101–5.0 × 104 cfu/mL. The lowest detection limit was found at 5 cfu/mL. Furthermore, the proposed immunosensor has been demonstrated with high sensitivity, good selectivity and reproducibility. Apparently, DGN-EIE may be a very useful tool for monitoring the bacteria.