Metabolic responses of Rhodococcus erythropolis PR4 grown on diesel oil and various hydrocarbons

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• 作者：Krisztián Laczi ; ágnes Kis ; Balázs Horváth…
• 关键词：Rhodococcus erythropolis PR4 ; Simultaneous hydrocarbon biodegradation ; Transcriptomics ; Diesel oil decomposition ; Metabolic response ; Oxygenases
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：99
• 期：22
• 页码：9745-9759
• 全文大小：1,027 KB
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• 作者单位：Krisztián Laczi (1)
  ágnes Kis (1)
  Balázs Horváth (3)
  Gergely Maróti (3)
  Botond Hegedüs (1)
  Katalin Perei (1)
  Gábor Rákhely (1) (2)
  
  1. Department of Biotechnology, University of Szeged, K?zép fasor 52, H-6726, Szeged, Hungary
  3. Institute of Biochemistry, Biological Research Centre Hungarian Academy of Sciences, Temesvári krt 62, H-6726, Szeged, Hungary
  2. Institute of Biophysics, Biological Research Centre Hungarian Academy of Sciences, Temesvári krt 62, H-6726, Szeged, Hungary
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Rhodococcus erythropolis PR4 is able to degrade diesel oil, normal-, iso- and cycloparaffins and aromatic compounds. The complete DNA content of the strain was previously sequenced and numerous oxygenase genes were identified. In order to identify the key elements participating in biodegradation of various hydrocarbons, we performed a comparative whole transcriptome analysis of cells grown on hexadecane, diesel oil and acetate. The transcriptomic data for the most prominent genes were validated by RT-qPCR. The expression of two genes coding for alkane-1-monooxygenase enzymes was highly upregulated in the presence of hydrocarbon substrates. The transcription of eight phylogenetically diverse cytochrome P450 (cyp) genes was upregulated in the presence of diesel oil. The transcript levels of various oxygenase genes were determined in cells grown in an artificial mixture, containing hexadecane, cycloparaffin and aromatic compounds and six cyp genes were induced by this hydrocarbon mixture. Five of them were not upregulated by linear and branched hydrocarbons. The expression of fatty acid synthase I genes was downregulated by hydrocarbon substrates, indicating the utilization of external alkanes for fatty acid synthesis. Moreover, the transcription of genes involved in siderophore synthesis, iron transport and exopolysaccharide biosynthesis was also upregulated, indicating their important role in hydrocarbon metabolism. Based on the results, complex metabolic response profiles were established for cells grown on various hydrocarbons. Our results represent a functional annotation of a rhodococcal genome, provide deeper insight into molecular events in diesel/hydrocarbon utilization and suggest novel target genes for environmental monitoring projects. Keywords Rhodococcus erythropolis PR4 Simultaneous hydrocarbon biodegradation Transcriptomics Diesel oil decomposition Metabolic response Oxygenases