Molecular dynamics simulation of a psychrophilic adenylate kinase

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• 作者：Euiyoung Bae (1) (2) (4)
  Sojin Moon (1)
  George N. Phillips Jr. (2) (3)
  
  1. Department of Agricultural Biotechnology ; Seoul National University ; Seoul ; 151-921 ; Republic of Korea
  2. Department of Biochemistry ; University of Wisconsin-Madison ; Madison ; WI ; 53706 ; USA
  4. Center for Food and Bioconvergence ; and Research Institute of Agriculture and Life Sciences ; Seoul National University ; Seoul ; 151-921 ; Republic of Korea
  3. Department of Biochemistry and Cell Biology ; Rice University ; Houston ; TX ; 77005 ; USA
  
• 关键词：Adenylate kinase ; Enzyme ; Molecular dynamics simulation ; Protein ; Psychrophile
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：58
• 期：2
• 页码：209-212
• 全文大小：280 KB
• 参考文献：1. Bae, E, Phillips, GN (2004) Structures and analysis of highly homologous psychrophilic, mesophilic, and thermophilic adenylate kinases. J Biol Chem 279: pp. 28202-28208 65200" target="_blank" title="It opens in new window">CrossRef
  2. Bae, E, Phillips, GN (2005) Identifying and engineering ion pairs in adenylate kinases. Insights from molecular dynamics simulations of thermophilic and mesophilic homologues. J Biol Chem 280: pp. 30943-30948 6200" target="_blank" title="It opens in new window">CrossRef
  3. Bae, E, Phillips, GN (2006) Roles of static and dynamic domains in stability and catalysis of adenylate kinase. Proc Natl Acad Sci USA 103: pp. 2132-2137 CrossRef
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• 刊物主题：Applied Microbiology; Bioorganic Chemistry; Biological Techniques;
• 出版者：Springer Netherlands
• ISSN：2234-344X

文摘

Proteins from psychrophilic organisms can function at low temperatures and have shown potential for industrial applications. In the present study, thermal denaturation of a psychrophilic adenylate kinase (AKpsychro) from Bacillus globisporus was measured using circular dichroism spectroscopy and its molecular dynamics (MD) simulations were performed. The results from the unfolding experiment and MD simulations of AKpsychro were compared with those of its mesophilic and thermophilic homologues to study the relationship between dynamic motion and the cold adaptation of proteins.