Fluorogenic Tagging Methodology Applied to Characterize Oxidized Tyrosine and Phenylalanine in an Immunoglobulin Monoclonal Antibody

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• 作者：Shuxia Zhou (1)
  Olivier Mozziconacci (1)
  Bruce A. Kerwin (2)
  Christian Sch?neich (1)
  
• 关键词：ABS fluorogenic tagging/derivatization ; immunoglobulin G (IgG) monoclonal antibody (mAb) ; metal catalyzed oxidation (MCO) ; protein degradation ; reverse phase liquid chromatography with mass spectrometry (RPLC ; MS)
• 刊名：Pharmaceutical Research
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：30
• 期：5
• 页码：1311-1327
• 全文大小：1071KB
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• 作者单位：Shuxia Zhou (1)
  Olivier Mozziconacci (1)
  Bruce A. Kerwin (2)
  Christian Sch?neich (1)
  
  1. Department of Pharmaceutical Chemistry, University of Kansas, 2095 Constant Avenue, Lawrence, Kansas, 66047, USA
  2. Department of Process and Product Development, Amgen Inc., 1201 Amgen Court West, Seattle, Washington, 98119, USA
  
• ISSN：1573-904X

文摘

Purpose Metal-catalyzed oxidation (MCO) of proteins is of primary concern in the development of biotherapeutics as it represents a prominent degradation pathway with potential undesired biological and biotherapeutic consequences. Methods We developed a fluorogenic derivatization methodology to study the MCO of IgG1 using a model oxidation system, CuCl2/L-ascorbic acid. Results Besides the oxidation of Met, Trp and His residues, we detected significant oxidation of Phe and Tyr in IgG1. Conclusion The fluorogenic derivatization method provides an alternative approach for the rapid detection of oxidized Tyr and Phe as their benzoxazole derivatives by fluorescence spectrometry and size exclusion chromatography coupled to fluorescence detection.