The effect of mechanical loading on osteogenesis of human dental pulp stromal cells in a novel in vitro model

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• 作者：Jun Ji ; Weibin Sun ; Wenmei Wang ; Theresa Munyombwe…
• 关键词：Biomimetic bioreactor ; Mechanical stimulation ; Biting force ; hDPSCs ; Bone tissue engineering ; Osteogenesis ; In vitro model
• 刊名：Cell and Tissue Research
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：358
• 期：1
• 页码：123-133
• 全文大小：1,734 KB
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• 作者单位：Jun Ji (1) (2) (3)
  Weibin Sun (1) (3)
  Wenmei Wang (1) (3)
  Theresa Munyombwe (4)
  Xuebin B. Yang (2) (3) (5)
  
  1. Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, 210008, People’s Republic of China
  2. Biomaterials and Tissue Engineering Group, School of Dentistry, University of Leeds, Leeds, LS2 9LU, UK
  3. Nanjing-Leeds Joint Centre for Oral Health Science, No.30 Zhongyang Road, Nanjing, 210008, People’s Republic of China
  4. Centre for Epidemiology & Biostatistics, University of Leeds, LS2 9LU, Leeds, UK
  5. NIHR Leeds Musculoskeletal Biomedical Research Unit, Chapel Allenton Hospital, Leeds, LS7 4SA, UK
  
• ISSN：1432-0878

文摘

Tooth loss often results in alveolar bone resorption because of lack of mechanical stimulation. Thus, the mechanism of mechanical loading on stem cell osteogenesis is crucial for alveolar bone regeneration. We have investigated the effect of mechanical loading on osteogenesis in human dental pulp stromal cells (hDPSCs) in a novel in vitro model. Briefly, 1?×-07 hDPSCs were seeded into 1?ml 3?% agarose gel in a 48-well-plate. A loading tube was then placed in the middle of the gel to mimic tooth-chewing movement (1?Hz, 3?×-0?min per day, n--). A non-loading group was used as a control. At various time points, the distribution of live/dead cells within the gel was confirmed by fluorescence markers and confocal microscopy. The correlation and interaction between the factors (e.g. force, time, depth and distance) were statistically analysed. The samples were processed for histology and immunohistochemistry. After 1- weeks of culture in the in-house-designed in vitro bioreactor, fluorescence imaging confirmed that additional mechanical loading increased the viable cell numbers over time as compared with the control. Cells of various phenotypes formed different patterns away from the reaction tube. The cells in the middle part of the gel showed enhanced alkaline phosphatase staining at week 1 but reduced staining at weeks 2 and 3. Additional loading enhanced Sirius Red and type I collagen staining compared with the control. We have thus successfully developed a novel in-house-designed in vitro bioreactor mimicking the biting force to enhance hDPSC osteogenesis in an agarose scaffold and to promote bone formation and/or prevent bone resorption.