A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences

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• 作者：Juan Li ; Fen Chen ; Hiromu Sugiyama ; David Blair ; Rui-Qing Lin…
• 关键词：Schistosoma japonicum ; Schistosomiasis ; Mitochondrial (mt) genome ; The Philippines ; Indel marker ; Comparative analyses ; Phylogeny
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：114
• 期：7
• 页码：2697-2704
• 全文大小：563 KB
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• 作者单位：Juan Li (1) (2)
  Fen Chen (3)
  Hiromu Sugiyama (4)
  David Blair (5)
  Rui-Qing Lin (6)
  Xing-Quan Zhu (2)
  
  1. Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong Province, People’s Republic of China
  2. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, 730046, Gansu Province, People’s Republic of China
  3. Department of Basic Medicine, Xiangnan University, Chenzhou, 423000, Hunan Province, People’s Republic of China
  4. Department of Parasitology, National Institute of Infectious Diseases, Tokyo, 113-8421, Japan
  5. School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, 4811, Australia
  6. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong Province, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

In the present study, near-complete mitochondrial (mt) genome sequences for Schistosoma japonicum from different regions in the Philippines and Japan were amplified and sequenced. Comparisons among S. japonicum from the Philippines, Japan, and China revealed a geographically based length difference in mt genomes, but the mt genomic organization and gene arrangement were the same. Sequence differences among samples from the Philippines and all samples from the three endemic areas were 0.57-.12 and 0.76-.85?%, respectively. The most variable part of the mt genome was the non-coding region. In the coding portion of the genome, protein-coding genes varied more than rRNA genes and tRNAs. The near-complete mt genome sequences for Philippine specimens were identical in length (14,091?bp) which was 4?bp longer than those of S. japonicum samples from Japan and China. This indel provides a unique genetic marker for S. japonicum samples from the Philippines. Phylogenetic analyses based on the concatenated amino acids of 12 protein-coding genes showed that samples of S. japonicum clustered according to their geographical origins. The identified mitochondrial indel marker will be useful for tracing the source of S. japonicum infection in humans and animals in Southeast Asia.