Development of a fluorescence–based multiplex genotyping method for simultaneous determination of human papillomavirus infections and viral loads
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- 作者：Zhengrong Sun ; Rong Zhang ; Zhonghua Liu ; Chao Liu ; Xiulin Li ; Weiqiang Zhou…
- 关键词：HPV ; BMRT ; GenoArray test ; Sequence ; Cervical lesion
- 刊名：BMC Cancer
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：15
- 期：1
- 全文大小：851 KB
- 刊物主题：Cancer Research; Oncology; Stem Cells; Animal Models; Internal Medicine;
- 出版者：BioMed Central
- ISSN：1471-2407
文摘
Background Persistent high-risk human papillomavirus (HPV) infection is correlated with an increased risk of developing intraepithelial lesion or malignancy (NILM). The aims of the current study is to establish a method named BioPerfectus Multiplex Real Time (BMRT) HPV assay for simultaneous typing and quantifying HPVs, and to evaluate it by comparison with HPV GenoArray test and PCR-sequencing method, as well as histological status.