What is the role of bosentan in healing of femur fractures in a rat model?

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• 作者：Ali Aydin ; Zekai Halici ; Erol Akpinar…
• 关键词：Bosentan ; Endothelin聽1 ; Bone fracture ; Rat ; Femur
• 刊名：Journal of Bone and Mineral Metabolism
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：33
• 期：5
• 页码：496-506
• 全文大小：1,740 KB
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• 作者单位：Ali Aydin (1)
  Zekai Halici (2)
  Erol Akpinar (2)
  A. Murat Aksakal (3)
  Murat Saritemur (4)
  Muhammed Yayla (2)
  C. Semih Kunak (5)
  Elif Cadirci (6)
  H. Tarik Atmaca (7)
  S. Sena Karcioglu (2)
  
  1. Department of Orthopedics and Traumatology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey
  2. Department of Pharmacology, Ataturk University Faculty of Medicine, 25240, Erzurum, Turkey
  3. Department of Orthopedics and Traumathology, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey, 16230
  4. Department of Emergency Medicine, Ataturk University Faculty of Pharmacy, 25240, Erzurum, Turkey
  5. Department of Pharmacology, Ordu University Faculty of Medicine, 52240, Ordu, Turkey
  6. Department of Pharmacology, Ataturk University Faculty of Pharmacy, 25240, Erzurum, Turkey
  7. Department of Pathology, K谋r谋kkale University Faculty of Veterinary, 71240, K谋r谋kkale, Turkey
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Metabolic Diseases
  Orthopedics
  Internal Medicine
  
• 出版者：Springer Japan
• ISSN：1435-5604

文摘

The purpose of this study was to examine the effects bosentan (which is a strong vasoconstrictor) on bone fracture pathophysiology, and investigate the roles of the nonselective endothelin 1 receptor blocker bosentan on the bone fractures formed in rats through radiographic, histopathologic, and immunohistochemical methods. The rats were divided into three groups (six rats in each group): a femoral fracture control group, a femoral fracture plus bosentan at 50 mg/kg group, and a femoral fracture plus bosentan at 100 mg/kg group. The femoral fracture model was established by transversely cutting the femur at the midsection. After manual reduction, the fractured femur was fixed with intramedullary Kirschner wires. The radiographic healing scores of the bosentan 100 and 50 mg/kg groups were significantly better that those of the fracture control group. The fracture callus percent of new bone in the bosentan 100 mg/kg group was significantly greater than that in the control group. Also, semiquantitative analysis showed higher positive vascular endothelial growth factor and osteocalcin staining and lower positive endothelin receptor type A staining in the treatment groups than in the control group. Bosentan treatment also decreased tissue endothelin 1 expression relative to that in the fracture control group. As a result of our study, the protective effect of bosentan was shown in experimental femoral fracture healing in rats by radiographic, histopathologic, and molecular analyses. Keywords Bosentan Endothelin 1 Bone fracture Rat Femur