Authentication of Quercus Species According to Their n-Alkanes Profile by Off-Line Combination of High-Performance Liquid Chromatography and Gas Chromatography

详细信息    查看全文

• 作者：Faten Rabhi ; M. Narváez-Rivas ; Sadok Boukhchina ; M. León-Camacho
• 关键词：n ; Alkanes ; Quercus ilex ; Quercus suber ; HPLC ; Gas chromatography ; Mass spectrometry ; Pattern recognition
• 刊名：Food Analytical Methods
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：8
• 期：7
• 页码：1710-1717
• 全文大小：710 KB
• 参考文献：Coomans D, Massart DL, Kaufman L (1979) Optimization by statistical linear discriminant analysis in analytical chemistry. Anal Chim Acta 112:97-22View Article
  De Muth JE (1999) Basic statistic and pharmaceutical statistical applications, 1st edn. Chapman and Hall/CRC, New York
  Gamero-Pasadas A, Viera-Alcalde I, Rios JJ, Graciani Constante E, Vicario IM, León-Camacho M (2006) Characterization and quantification of the hydrocarbons fraction of the subcutaneous fresh fat of Iberian pig by off-line combination of high performance liquid chromatography and gas chromatography. J Chrom A 1123:82-1View Article
  Gardiner, W.P.(Ed).1997. Statistical analysis methods for chemistry, Royal Society of Chemistry. Cambridge. pp. 313-28.
  Guinda A, Lanzón A, Albi T (1996) Differences in hydrocarbons of virgin olive oils obtained from several olive varieties. J Agric Food Chem 44:1723-726View Article
  Harwood,J.L., R. Aparicio, R. (Eds.). 2013. Handbook of Olive Oil: Analysis and Properties, Springer, New York, pp 163-18
  http://?www.?pherobase.?com/?database/?kovats/?kovats-index.?php . 2012.
  International Union of Pure and Applied Chemistry (IUPAC) (1987) Method 1122. Determination of oil content (extraction method). In: Paquot C, Haufenne A (eds) Standard methods for the analysis of oils, fats and derivatives, 7th edn. Blackwell Scientific Publications, Oxford, pp 14-6
  Jolliffe IT (2002) Principal components analysis, 2nd edn. Springer, New York
  Lanzón A, Albi T, Cert A, Gracián J (1994) The hydrocarbon fraction of virgin olive oil and changes resulting from refining. JAOCS 71:285-92
  Nolan JV, Leng RA, Demeyer DI (eds) (1989) The roles of protozoa and fungi in ruminant digestion. Pernanbul Books, New South Whales, pp 153-67
  Silva SP, Sabino MA, Fernandes EM, Correlo VM, Boesel LF, Reis RL (2005) Cork: properties, capabilities and applications. Int Mater Rev 50:345-65View Article
  Soto E, Hoz L, Ordonez JA, Hierro E, Herranz B, Lopez-Bote C, Cambero MI (2008) Impact of feeding and rearing systems of Iberian pigs on volatile profile and sensory characteristics of dry-cured loin. Meat Sci 79:666-76View Article
  StatSoft Inc. 1995. Statistica 5.5 for Windows Computer Program Manual, Tulsa, OK. StatSoft, Inc., 1995, 2300 East 14th Street, Tulsa, OK 74104
  Toumi L, Lumaret R (2001) Allozyme characterisation of four Mediterranean evergreen oak species. Biochem Syst Ecol 29:799-17View Article
  Waghorn GC (1990) Effect of condensed tannins on protein digestion and nutritive value of fresh herbage. Hoc Aust Soc Anim Prod 18:412-15
  Webster L, Simpson P, Shanks AM, Moffat CF (2000) The authentication of olive oil on the basis of hydrocarbon concentration and composition. Analyst 125:97-04View Article
  
• 作者单位：Faten Rabhi (1)
  M. Narváez-Rivas (2)
  Sadok Boukhchina (1)
  M. León-Camacho (2)
  
  1. Lipids Biochemistry Laboratory, Biology Department, Faculty of Science, Tunis El-Manar University, Tunis, Tunisia
  2. Food Characterization and Quality Department, Instituto de la Grasa (C.S.I.C.), Avda. Padre García Tejero, 4, 41012, Seville, Spain
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X

文摘

n-Alkanes of 15 samples of acorn oil from Quercus ilex and Quercus suber have been analysed by gas chromatography. Oil was extracted in a Soxhlet glass apparatus using petroleum ether as solvent. The unsaponifiable matter was fractionated by high-performance liquid chromatography. Then, the analysis was performed on a capillary DB-5MS column (30?m?×-.25?mm i.d., 0.25?μm film thickness), with hydrogen as carrier gas and using a flame ionization detector. Eicosane was used as internal standard for quantification of individual n-alkanes. Gas chromatography-mass spectrometry (GC-MS) analysis in full scan mode was used for their identification. Ten compounds have been identified in these samples: heneicosane, docosane, tricosane, tetracosane, pentacosane, hexacosane, heptacosane, octacosane, nonacosane and triacontane. By using these compounds as chemical descriptors, pattern recognition techniques, such as principal component analysis and linear discriminant analysis, were applied to find out a suitable classification model and differentiate between Q. ilex and Q. suber genuses. The results showed that these compounds allowed the differentiation of the two genuses with a complete classification.